| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACTN4 |
| Uniprot No | O43707 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 397-648aa |
| 氨基酸序列 | EEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNE |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Role of ACTN4 in Tumor Cell Motility and Invasion"** by Honda et al.
- 研究通过体外实验证实重组ACTN4蛋白通过增强肌动蛋白交联促进癌细胞的迁移和侵袭能力，与多种癌症转移相关。
2. **"ACTN4 Mutations Cause Focal Segmental Glomerulosclerosis"** by Kaplan et al.
- 发现ACTN4基因突变导致其重组蛋白功能异常，引发肾小球足细胞损伤，与家族性局灶节段性肾小球硬化症(FSGS)发病机制相关。
3. **"Structural Insights into ACTN4-Actin Interaction"** by Korematsu & Ishizaki
- 通过X射线晶体学解析重组ACTN4蛋白与肌动蛋白的结合结构，揭示其CH结构域的关键作用，为靶向药物设计提供依据。
4. **"ACTN4 as a Biomarker in Ovarian Cancer"** by Smith et al.
- 临床研究表明，重组ACTN4蛋白在卵巢癌组织中的过表达与患者预后不良显著相关，提示其作为潜在诊断标志物的价值。
**Background of ACTN4 Recombinant Protein**
ACTN4 (alpha-actinin-4) is a cytoskeletal protein belonging to the α-actinin family, which plays a critical role in maintaining cell structure, motility, and adhesion. It crosslinks actin filaments, stabilizing the cytoskeleton and regulating cellular mechanics. Unlike other isoforms, ACTN4 is predominantly expressed in non-muscle cells and localizes to dynamic structures like lamellipodia and focal adhesions, where it modulates cell migration and signaling pathways.
The ACTN4 gene is evolutionarily conserved and associated with human diseases. Mutations in ACTN4 are linked to focal segmental glomerulosclerosis (FSGS), a kidney disorder characterized by podocyte dysfunction and proteinuria. Additionally, ACTN4 overexpression correlates with cancer progression, particularly in enhancing tumor cell invasion and metastasis through interactions with transcription factors (e.g., NF-κB) and cytoskeletal remodeling.
Recombinant ACTN4 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactions, and disease mechanisms. Purified recombinant ACTN4 retains functional domains, including the actin-binding N-terminus, spectrin-like repeats, and a C-terminal calmodulin-like domain, enabling *in vitro* studies on actin binding, protein-protein interactions, and mutational effects.
Research applications include elucidating ACTN4's role in cellular dynamics, validating its pathological mutations, and screening therapeutic agents targeting ACTN4-associated pathways. Its recombinant form also aids in developing diagnostic tools for ACTN4-related disorders. Overall, ACTN4 recombinant protein serves as a vital tool for understanding cytoskeletal regulation and its implications in disease.
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