| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMD2 |
| Uniprot No | Q13200 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-908aa |
| 氨基酸序列 | MEEGGRDKAPVQPQQSPAAAPGGTDEKPSGKERRDAGDKDKEQELSEEDKQLQDELEMLVERLGEKDTSLYRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKRFAADIISVLAMTMSGERECLKYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKVQREPLLTLVKEIVPYNMAHNAEHEACDLLMEIEQVDMLEKDIDENAYAKVCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVEDIFTSCKDVVVQKQMAFMLGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYKTHLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLWDVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYAGSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETELKDTYARWLPLGLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHLGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPLPVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYDL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSMD2重组蛋白的3篇示例参考文献(注：示例为虚构内容，实际文献需通过学术数据库查询)：
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1. **文献名称**：*"Recombinant PSMD2 Protein Expression and Structural Characterization in Human Cancer Cells"*
**作者**：Chen L, et al.
**摘要**：本研究成功在大肠杆菌系统中表达了重组PSMD2蛋白，并通过亲和层析纯化获得高纯度产物。利用圆二色谱和X射线晶体学解析了PSMD2的C端结构，揭示了其与蛋白酶体亚基PSMD1的相互作用界面，为靶向泛素-蛋白酶体通路的药物开发提供依据。
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2. **文献名称**：*"Functional Analysis of PSMD2 Knockdown and Recombinant Rescue in Colorectal Cancer Proliferation"*
**作者**：Wang Y, et al.
**摘要**：通过siRNA敲低PSMD2基因显著抑制结直肠癌细胞增殖，而外源添加重组PSMD2蛋白可部分恢复细胞活性。实验表明PSMD2通过调控p53泛素化降解通路影响肿瘤生长，提示其作为潜在治疗靶点。
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3. **文献名称**：*"Development of a PSMD2-Based ELISA Assay for Proteasome Activity Screening"*
**作者**：Kim S, et al.
**摘要**：研究利用重组PSMD2蛋白作为捕获抗原，开发了一种新型ELISA检测方法，可定量分析细胞裂解液中26S蛋白酶体的组装状态。该方法在阿尔茨海默病模型小鼠脑组织中验证了蛋白酶体活性异常。
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**建议**：实际研究中可查阅PubMed、Web of Science等平台，以关键词“PSMD2 recombinant protein”“proteasome subunit”等检索近期文献，重点关注功能机制或疾病关联研究。
**Background of PSMD2 Recombinant Protein**
PSMD2 (Proteasome 26S Subunit, Non-ATPase 2) is a critical component of the 19S regulatory particle of the 26S proteasome, a large multi-subunit complex responsible for targeted protein degradation in eukaryotic cells. As part of the ubiquitin-proteasome system (UPS), PSMD2 plays a vital role in recognizing polyubiquitinated substrates, facilitating their deubiquitination, and directing them to the proteolytic core for degradation. This process is essential for maintaining cellular homeostasis, regulating cell cycle progression, DNA repair, and stress responses. Dysregulation of PSMD2 or the proteasome is linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
Recombinant PSMD2 protein is produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, enabling high-yield, pure protein for functional and structural studies. Its production often involves tagging (e.g., His-tag) for simplified purification via affinity chromatography. Researchers utilize PSMD2 recombinant protein to investigate proteasome assembly, substrate recognition mechanisms, and interactions with other regulatory components or inhibitors. It also serves as a tool for screening potential therapeutics targeting the UPS, particularly in diseases where proteasome activity is aberrant.
Moreover, studies leveraging recombinant PSMD2 contribute to understanding its role in modulating proteasome activity under stress conditions or in disease models. Its application extends to structural biology (e.g., cryo-EM studies) and developing assays to measure proteasomal activity or inhibitor efficacy. As the UPS remains a key therapeutic target, PSMD2 recombinant protein is pivotal in advancing both basic research and drug discovery efforts.
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