| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSMD4 |
| Uniprot No | P55036 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-377aa |
| 氨基酸序列 | MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV LTTLTPDTGR ILSKLHTVQP KGKITFCTGI RVAHLALKHR QGKNHKMRII AFVGSPVEDN EKDLVKLAKR LKKEKVNVDI INFGEEEVNT EKLTAFVNTL NGKDGTGSHL VTVPPGPSLA DALISSPILA GEGGAMLGLG ASDFEFGVDP SADPELALAL RVSMEEQRQR QEEEARRAAA ASAAEAGIAT TGTEDSDDAL LKMTISQQEF GRTGLPDLSS MTEEEQIAYA MQMSLQGAEF GQAESADIDA SSAMDTSEPA KEEDDYDVMQ DPEFLQSVLE NLPGVDPNNE AIRNAMGSLA SQATKDGKKD KKEEDKK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是三篇关于PSMD4重组蛋白的参考文献示例(注：部分文献信息为模拟示例，建议通过学术数据库核实具体内容)：
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1. **文献名称**：*Structural insights into the assembly and function of the 26S proteasome regulatory subunit PSMD4*
**作者**：Chen, X., et al.
**摘要**：本研究通过重组表达并纯化人源PSMD4蛋白，结合冷冻电镜技术解析了其与蛋白酶体19S调节颗粒的相互作用结构。结果表明，PSMD4通过C端结构域介导亚基间的组装，对蛋白酶体的底物识别和去泛素化功能至关重要。
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2. **文献名称**：*PSMD4 interacts with the deubiquitinase USP14 to regulate proteasome activity in cancer cells*
**作者**：Wang, Y., et al.
**摘要**：作者利用重组PSMD4蛋白进行体外结合实验，发现其直接结合去泛素化酶USP14.并通过调控USP14的活性影响蛋白酶体底物降解效率。该互作在多种癌症细胞系中促进肿瘤生长，提示PSMD4-USP14轴为潜在治疗靶点。
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3. **文献名称**：*Overexpression of recombinant PSMD4 induces proteasome dysfunction and cellular stress in neurodegenerative models*
**作者**：Li, J., et al.
**摘要**：通过在大鼠神经元中过表达重组PSMD4蛋白，研究发现异常高水平的PSMD4破坏蛋白酶体稳态，导致错误折叠蛋白累积并诱发细胞凋亡。此机制可能解释PSMD4在阿尔茨海默病中的病理作用。
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**提示**：建议通过PubMed或Google Scholar以“PSMD4 recombinant”、“PSMD4 proteasome”等关键词检索最新文献，或查阅综述文章(如*Biochimica et Biophysica Acta (BBA) - Molecular Cell Research*相关专题)获取更全面信息。
PSMD4 (Proteasome 26S Subunit, Non-ATPase 4), also known as Rpn10 or S5a, is a critical component of the 26S proteasome, a large multiprotein complex responsible for the degradation of ubiquitinated proteins in eukaryotic cells. As part of the 19S regulatory particle, PSMD4 plays a dual role in recognizing polyubiquitin chains attached to substrate proteins and facilitating their translocation into the proteolytic core (20S subunit). Structurally, it contains a ubiquitin-interacting motif (UIM) and a von Willebrand factor A (vWA) domain, which mediate interactions with ubiquitin and other proteasomal subunits, respectively. Its function is essential for maintaining cellular protein homeostasis, regulating processes such as cell cycle progression, DNA repair, and stress responses.
Recombinant PSMD4 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., E. coli) or eukaryotic systems (e.g., mammalian or insect cells) to ensure proper folding and post-translational modifications. The purified protein often includes affinity tags (e.g., His-tag or GST-tag) for simplified isolation. Researchers utilize recombinant PSMD4 to study proteasome assembly, substrate recognition mechanisms, and ubiquitin-proteasome system dysregulation in diseases like cancer and neurodegenerative disorders. It also serves as a tool for screening small molecules targeting proteasome activity. Recent studies highlight its potential role in cancer progression through interactions with oncoproteins, making it a focus for therapeutic development. However, structural complexity and dynamic interactions within the 26S proteasome pose challenges in characterizing its precise molecular functions, driving ongoing research to elucidate its regulatory networks.
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