纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | NME5 |
Uniprot No | P56597 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-212aa |
氨基酸序列 | MEISMPPPQI YVEKTLAIIK PDIVDKEEEI QDIILRSGFT IVQRRKLRLS PEQCSNFYVE KYGKMFFPNL TAYMSSGPLV AMILARHKAI SYWLELLGPN NSLVAKETHP DSLRAIYGTD DLRNALHGSN DFAAAEREIR FMFPEVIVEP IPIGQAAKDY LNLHIMPTLL EGLTELCKQK PADPLIWLAD WLLKNNPNKP KLCHHPIVEE PY |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NME5重组蛋白的3条示例参考文献(内容为虚构,供参考):
1. **文献名称**:Cloning and Functional Characterization of Recombinant NME5 in Cancer Cell Metastasis
**作者**:Li X, Zhang Y, et al.
**摘要**:研究通过大肠杆菌系统成功表达NME5重组蛋白,发现其通过调控细胞能量代谢抑制乳腺癌细胞迁移,提示其在肿瘤转移中的潜在抑制作用。
2. **文献名称**:Structural Insights into NME5 Recombinant Protein and Its Interaction with Tubulin
**作者**:Wang H, Kumar S, et al.
**摘要**:利用X射线晶体学解析NME5重组蛋白的三维结构,揭示其与微管蛋白的结合位点,为理解其在细胞骨架动态调节中的作用提供结构基础。
3. **文献名称**:NME5 Recombinant Protein Attenuates Oxidative Stress in Neuronal Cells
**作者**:Chen L, Rodriguez-Mateos A, et al.
**摘要**:研究发现重组NME5蛋白通过激活抗氧化通路(如Nrf2)显著减少神经元氧化损伤,提示其在中枢神经系统疾病治疗中的应用潜力。
注:以上文献信息为模拟生成,实际研究中需以真实数据库(如PubMed)检索为准。
**Background of NME5 Recombinant Protein**
NME5 (non-metastatic protein 5), also known as NDPK-related kinase 5 or NM23-H5. is a member of the NM23/nucleoside diphosphate kinase (NDPK) family. This family is evolutionarily conserved and primarily involved in cellular processes such as nucleotide metabolism, metastasis suppression, and DNA repair. Unlike other NM23 family members (e.g., NME1-4), NME5 lacks canonical NDPK enzymatic activity due to structural variations in its active site. Instead, it functions as a regulatory protein with roles in ciliogenesis, spermatogenesis, and cellular stress responses.
Structurally, NME5 forms hexamers and interacts with other proteins, such as NME6. to form heterohexamers. It localizes to the cytoplasm, nucleus, and cilia, suggesting diverse functional contexts. Studies highlight its importance in primary cilia formation, where it regulates microtubule dynamics and signaling pathways (e.g., Hedgehog signaling). In reproduction, NME5 is critical for sperm flagellar stability, and its deficiency is linked to male infertility in animal models.
The recombinant NME5 protein is engineered using expression systems like *E. coli* or mammalian cells, often tagged with fusion partners (e.g., His-tag) for purification. Its production enables biochemical studies, including structural analysis, interaction mapping, and functional assays. Researchers use recombinant NME5 to explore its role in diseases such as cancer (as a putative metastasis suppressor), ciliopathies, and reproductive disorders. Additionally, it serves as a tool for screening therapeutic agents targeting cilia-related pathologies or fertility issues.
Despite progress, NME5’s precise mechanisms remain partially unresolved. Ongoing research aims to clarify its non-enzymatic signaling roles and potential as a biomarker or therapeutic target, emphasizing its unique position within the NM23 family.
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