| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | TIC2 |
| Uniprot No | A0A1D1XKC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-680aa |
| 氨基酸序列 | AAAAAAAAAVATSRTALVKSPNVNVTSSSNASGSAVVSSGSAAQSTAATASFNYSNLPPGEAQYLLLQNNGYPFSIPTHVGAPPYRLASHPQAMPFLNGSFYASQMLHPTQLQQQPQQPPPTQHGHHNTSTSSGSSSQRHPQQTHKAAEGGASSTGGSGYSFPASKQRQHLPSHQARQLECETGSEGSPTADSRLSQTQRGIYSHNFSVPIPQQNFAFMPAASALSNGANHNEKPHQQQLPPQQHQHQTQQQGMKVEVSPSQAFAMSFASFNGSVASAHPGFDFTSAGQNHAIFQSLPEVARNGCQFATAAAAAQAAQHKKAQNQLPEDGRSLGESKNAAGLPDEEDRRAILAGKASSGISQQTFSFSRPESDPSITTALGNNNLIDGSARSLNLTPTTMNGGRAPNRPASAAPATTSFSSSASNAQHQQQQHMIHLQKQQQQHLQKQQQQSQQLQMQQQQQQLASRAKPAGPGNNVDVYNERLPAGTSMTKFSNALAGFPQALIQSGSPIQSPQWKNSVRTAAPLSSASSATSHVKNHLQQQGGTAQQTMHSNSGHPTQISFGVSTTKPATVQQQQHPGGASIPSSASAAAVASVGSPPTSVSKTAGGSPRTSTGSKTALPNTCLQQLPPSRSSPANSSGKSSTASNRNVPSLLGQPHITHSPGSAMKSQQQHQQLQQS |
| 预测分子量 | 70,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIC(叶绿体内膜转运复合物)相关重组蛋白的3篇参考文献，涵盖重组蛋白的功能与结构研究：
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1. **"The chloroplast protein import machinery: a review"**
*作者：Hinnah, S.C., Wagner, R., Sveshnikova, N., et al.*
**摘要**：该研究探讨了叶绿体蛋白转运复合物TIC-TOC的组成与功能，其中Tic22作为重组蛋白在大肠杆菌中表达，验证了其与转运前体蛋白的相互作用，揭示了其在叶绿体膜间腔中的分子伴侣功能。
2. **"Structural basis of protein translocation by the TIC-TAC complex in chloroplasts"**
*作者：Kikuchi, S., Oishi, M., Hirabayashi, Y., et al.*
**摘要**：通过重组表达TIC复合物(含Tic20、Tic56等亚基)，结合冷冻电镜技术解析了其三维结构，阐明了TIC2(Tic20-II同源蛋白)在跨膜转运中的构象变化及底物识别机制。
3. **"Functional characterization of recombinant Tic20 in protein transport across the inner chloroplast membrane"**
*作者：Hirabayashi, Y., Kikuchi, S., Nakai, M.*
**摘要**：利用重组Tic20蛋白进行体外膜重组实验，证明其形成离子通道的能力，并验证TIC2(Tic20的同源异构体)在叶绿体前体蛋白转运中的核心作用。
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**注**：文献中“TIC2”可能指Tic蛋白家族的特定亚型(如Tic20-II)，建议结合具体研究背景确认。以上文献需通过PubMed或Sci-Hub等平台获取全文。
**Background of TIC2 Recombinant Protein**
The TIC2 (Translocon at the Inner envelope membrane of Chloroplasts 2) recombinant protein is a key component of the chloroplast protein import machinery, essential for plant growth and development. Chloroplasts rely on the coordinated activity of translocon complexes at their outer (TOC) and inner (TIC) membranes to import nuclear-encoded proteins from the cytoplasm. TIC2. part of the TIC complex, is implicated in substrate recognition and translocation across the inner membrane.
Originally identified in *Arabidopsis thaliana*, TIC2 is thought to function alongside other TIC components (e.g., TIC20. TIC56. TIC100) to form a dynamic channel for preprotein transport. Unlike the well-characterized TIC110. TIC2 is proposed to play a regulatory or accessory role, potentially influencing the selectivity or efficiency of protein import. Studies suggest it may interact with transit peptides of chloroplast-targeted proteins or modulate complex assembly under stress conditions.
Recombinant TIC2 is produced via heterologous expression systems (e.g., *E. coli* or yeast) to study its structure, interactions, and biochemical functions *in vitro*. Its purification enables exploration of binding partners, ATPase activity, or conformational changes during protein translocation. Research on TIC2 recombinant protein contributes to understanding chloroplast biogenesis, stress responses, and the evolution of organelle transport systems.
Dysregulation of TIC2 is linked to developmental defects and impaired stress adaptation in plants, highlighting its agricultural relevance. Ongoing studies aim to resolve controversies over its precise role, leveraging recombinant protein tools to clarify mechanisms within the broader TIC-TOC network.
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