| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMAN1 |
| Uniprot No | P49257 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-477aa |
| 氨基酸序列 | DGVGGDPAVALPHRRFEYKYSFKGPHLVQSDGTVPFWAHAGNAIPSSDQIRVAPSLKSQRGSVWTKTKAAFENWEVEVTFRVTGRGRIGADGLAIWYAENQGLEGPVFGSADLWNGVGIFFDSFDNDGKKNNPAIVIIGNNGQIHYDHQNDGASQALASCQRDFRNKPYPVRAKITYYQNTLTVMINNGFTPDKNDYEFCAKVENMIIPAQGHFGISAATGGLADDHDVLSFLTFQLTEPGKEPPTPDKEISEKEKEKYQEEFEHFQQELDKKKEEFQKGHPDLQGQPAEEIFESVGDRELRQVFEGQNRIHLEIKQLNRQLDMILDEQRRYVSSLTEEISKRGAGMPGQHGQITQQELDTVVKTQHEILRQVNEMKNSMSETVRLVSGMQHPGSAGGVYETTQHFIDIKEHLHIVKRDIDNLVQRNMPSNEKPKCPELPPFPSCLS |
| 预测分子量 | 57.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LMAN1重组蛋白的3篇代表性文献摘要简述：
1. **"Structural basis of LMAN1 in ER-to-Golgi transport"**
- **作者**: Zhang B. et al.
- **摘要**: 该研究解析了重组人源LMAN1蛋白的晶体结构，揭示了其N端凝集素结构域与货物蛋白(如凝血因子V/VIII)的糖基化依赖性结合机制，阐明了LMAN1在内质网到高尔基体运输中的分子作用。
2. **"LMAN1 functions as a cargo receptor for glycoprotein secretion"**
- **作者**: Nyfeler B. et al.
- **摘要**: 通过重组LMAN1蛋白的体外实验，证明其作为分子伴侣与凝血因子V/VVIII的特定糖链结合，并验证了其缺失导致凝血因子分泌缺陷的病理机制。
3. **"Recombinant LMAN1 restores protein trafficking in cellular models of LMAN1 deficiency"**
- **作者**: Hauri H.P. et al.
- **摘要**: 研究利用重组LMAN1蛋白在缺陷型细胞系中恢复ERGIC-53依赖的蛋白运输功能，证实了LMAN1在遗传性出血性疾病(如combined deficiency of F5F8D)中的关键作用。
如需具体文献来源或更多细节，建议通过PubMed/Google Scholar以关键词“LMAN1 recombinant protein”或“ERGIC-53 expression”检索近期研究。
LMAN1 (Lectin, Mannose Binding 1), also known as ERGIC-53. is a conserved transmembrane protein belonging to the C-type lectin family. It functions as a cargo receptor in the early secretory pathway, facilitating the transport of glycoproteins from the endoplasmic reticulum (ER) to the ER-Golgi intermediate compartment (ERGIC). Structurally, LMAN1 forms hexamers and binds mannose-rich glycans on target proteins via its carbohydrate recognition domain (CRD). Key clients include coagulation factors V and VIII, as well as alpha-1 antitrypsin. Mutations in LMAN1 are linked to combined deficiency of factors V and VIII (F5F8D), a rare autosomal recessive bleeding disorder, highlighting its critical role in hemostasis.
Recombinant LMAN1 protein is produced using expression systems like mammalian cells or bacteria, often tagged for purification and detection. This engineered protein enables detailed studies of LMAN1’s structure-function relationships, glycan-binding specificity, and interactions with cofactors such as MCFD2. Researchers use it to model intracellular trafficking mechanisms, investigate molecular pathologies of F5F8D, and explore therapeutic strategies like gene correction. Additionally, recombinant LMAN1 serves as a tool for structural biology (e.g., crystallography) and developing diagnostic assays for coagulation disorders. Its applications extend to studying viral entry mechanisms, as some pathogens exploit LMAN1 for host cell infection. Overall, recombinant LMAN1 is vital for advancing both basic and clinical research in glycoprotein trafficking and related diseases.
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