| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLSTN2 |
| Uniprot No | Q9H4D0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-955aa |
| 氨基酸序列 | MLPGRLCWVPLLLALGVGSGSGGGGDSRQRRLLAAKVNKHKPWIETSYHGVITENNDTVILDPPLVALDKDAPVPFAGEICAFKIHGQELPFEAVVLNKTSGEGRLRAKSPIDCELQKEYTFIIQAYDCGAGPHETAWKKSHKAVVHIQVKDVNEFAPTFKEPAYKAVVTEGKIYDSILQVEAIDEDCSPQYSQICNYEIVTTDVPFAIDRNGNIRNTEKLSYDKQHQYEILVTAYDCGQKPAAQDTLVQVDVKPVCKPGWQDWTKRIEYQPGSGSMPLFPSIHLETCDGAVSSLQIVTELQTNYIGKGCDRETYSEKSLQKLCGASSGIIDLLPSPSAATNWTAGLLVDSSEMIFKFDGRQGAKVPDGIVPKNLTDQFTITMWMKHGPSPGVRAEKETILCNSDKTEMNRHHYALYVHNCRLVFLLRKDFDQADTFRPAEFHWKLDQICDKEWHYYVINVEFPVVTLYMDGATYEPYLVTNDWPIHPSHIAMQLTVGACWQGGEVTKPQFAQFFHGSLASLTIRPGKMESQKVISCLQACKEGLDINSLESLGQGIKYHFNPSQSILVMEGDDIGNINRALQKVSYINSRQFPTAGVRRLKVSSKVQCFGEDVCISIPEVDAYVMVLQAIEPRITLRGTDHFWRPAAQFESARGVTLFPDIKIVSTFAKTEAPGDVKTTDPKSEVLEEMLHNLDFCDILVIGGDLDPRQECLELNHSELHQRHLDATNSTAGYSIYGVGSMSRYEQVLHHIRYRNWRPASLEARRFRIKCSELNGRYTSNEFNLEVSILHEDQVSDKEHVNHLIVQPPFLQSVHHPESRSSIQHSSVVPSIATVVIIISVCMLVFVVAMGVYRVRIAHQHFIQETEAAKESEMDWDDSALTITVNPMEKHEGPGHGEDETEGEEEEEAEEEMSSSSGSDDSEEEEEEEGMGRGRHGQNGARQAQLEWDDSTLPY |
| 预测分子量 | 107 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLSTN2重组蛋白的3篇参考文献的简要总结(注：文献为虚拟示例，实际研究中请核实真实来源)：
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1. **文献名称**: "Calsyntenin-2/CLSTN2 regulates excitatory synaptic transmission and network integration in hippocampal neurons"
**作者**: Müller, T., et al.
**摘要**: 本研究通过重组CLSTN2蛋白体外表达，发现其通过结合谷氨酸受体亚基(如GluA1)调控突触后膜受体运输，影响海马神经元的兴奋性突触传递及神经网络整合功能。
2. **文献名称**: "Structural insights into the calcium-dependent interaction between CLSTN2 and α-neurexins"
**作者**: Li, J., et al.
**摘要**: 利用重组CLSTN2胞外域蛋白进行X射线晶体学分析，揭示了其与突触黏附分子α-neurexin的钙依赖性结合机制，为CLSTN2在突触形成中的分子作用提供结构基础。
3. **文献名称**: "CLSTN2 recombinant protein attenuates Aβ-induced synaptic toxicity in Alzheimer's disease models"
**作者**: Park, S., et al.
**摘要**: 在阿尔茨海默病细胞模型中，外源性重组CLSTN2蛋白通过竞争性抑制β淀粉样蛋白(Aβ)与神经元膜的结合，减轻突触毒性，提示其潜在治疗价值。
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**备注**：以上内容为示例，实际文献需通过PubMed、Web of Science等平台检索关键词(如CLSTN2 recombinant, calsyntenin-2 expression)获取。
**Background of CLSTN2 Recombinant Protein**
CLSTN2 (Calsyntenin-2), also known as Alcadein-β, is a member of the calsyntenin protein family, which comprises neuron-enriched type I transmembrane proteins. These proteins are characterized by extracellular cadherin-like domains, a single transmembrane region, and a short cytoplasmic tail. CLSTN2 is predominantly expressed in the central nervous system (CNS), where it localizes to synaptic and vesicular compartments, suggesting roles in neuronal communication, intracellular trafficking, or synaptic organization.
Functionally, CLSTN2 interacts with components of the APP (amyloid precursor protein) complex, including X11/Mint proteins, and is implicated in modulating amyloid-β metabolism—a key pathway in Alzheimer’s disease pathogenesis. Studies also highlight its involvement in calcium homeostasis, vesicle transport, and synaptic plasticity, linking it to broader neurological processes and potential neurodegenerative disorders.
Recombinant CLSTN2 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional protein for *in vitro* studies. This tool enables researchers to dissect CLSTN2’s structural properties, ligand interactions, and signaling mechanisms. Applications range from biochemical assays (e.g., binding studies) to cellular models exploring its role in neuronal health or disease.
The development of CLSTN2 recombinant protein underscores its growing importance as a molecular probe in neuroscience research, particularly for understanding synaptic dysfunction and designing therapeutic strategies for neurological conditions.
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