| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLYT1 |
| Uniprot No | P26572 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPAPGRPPSVSALDGDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGRVPTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPAAVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPLTWEGYDPSWN |
| 预测分子量 | 50,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLYT1重组蛋白的参考文献示例，基于典型研究方向整理：
1. **"Structural Basis of Glycine Transport by GlyT1"**
*作者：Wang et al., 2021*
**摘要**：通过冷冻电镜解析人源GLYT1的三维结构，揭示了其与甘氨酸、钠离子及氯离子的结合位点，阐明了离子耦合转运的分子机制。
2. **"Functional Expression of Recombinant GLYT1 in HEK293 Cells for Pharmacological Profiling"**
*作者：López-Corcuera et al., 2017*
**摘要**：在HEK293细胞中重组表达GLYT1.验证其甘氨酸摄取功能，并用于评估多种抑制剂(如ALX5407)的效价及选择性。
3. **"Probing Allosteric Modulation of GLYT1 Using Fluorescence-Based Assays"**
*作者：Zhang et al., 2019*
**摘要**：开发基于重组GLYT1蛋白的荧光淬灭实验，筛选出新型变构调节剂，为精神分裂症治疗的药物开发提供候选分子。
4. **"Role of GLYT1 in Glycine Homeostasis: Insights from Knockout Models and Recombinant Systems"**
*作者：Giménez et al., 2020*
**摘要**：结合重组蛋白体外实验与基因敲除小鼠模型，证明GLYT1通过调控突触甘氨酸浓度影响NMDA受体活性及神经传递功能。
*注：上述文献为示例性质，实际引用时需核实具体文章是否存在及准确性。建议通过PubMed或Google Scholar以关键词“GLYT1 recombinant protein”搜索最新研究。*
**Background of GLYT1 Recombinant Protein**
Glycine transporter 1 (GLYT1), encoded by the *SLC6A9* gene, belongs to the solute carrier 6 (SLC6) family of neurotransmitter transporters. It plays a critical role in regulating extracellular glycine concentrations in the central nervous system (CNS) by mediating glycine reuptake from synaptic clefts into glial cells and presynaptic neurons. GLYT1 is predominantly expressed in the brainstem, spinal cord, and retina, where it fine-tunes glycinergic neurotransmission. Glycine itself acts as both an inhibitory neurotransmitter (via glycine receptors in the spinal cord) and a co-agonist of excitatory NMDA receptors (in forebrain regions), making GLYT1 a key modulator of synaptic plasticity and neural circuit activity.
Dysregulation of GLYT1 has been implicated in neurological and psychiatric disorders, including schizophrenia, neuropathic pain, and neurodegenerative diseases. Inhibiting GLYT1 to elevate synaptic glycine levels has emerged as a therapeutic strategy, particularly for enhancing NMDA receptor function in conditions like schizophrenia, where hypofunction of these receptors is observed.
Recombinant GLYT1 proteins are engineered in vitro using expression systems such as mammalian cells (e.g., HEK293) to produce functional, purified forms of the transporter for research. These proteins retain structural and functional properties, including 12 transmembrane domains, glycosylation sites, and sodium/chloride ion dependency for glycine transport. Tagged versions (e.g., His-tag, FLAG-tag) facilitate purification and detection.
GLYT1 recombinant proteins are widely used in drug discovery (e.g., screening small-molecule inhibitors), biochemical assays (binding kinetics, ion dependence), and structural studies (cryo-EM, X-ray crystallography) to elucidate transport mechanisms and ligand interactions. Their application advances the development of targeted therapies for glycine-related CNS disorders, highlighting their importance in both basic neuroscience and translational medicine.
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