| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAN1A1 |
| Uniprot No | P33908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-653aa |
| 氨基酸序列 | MPVGGLLPLFSSPAGGVLGGGLGGGGGRKGSGPAALRLTEKFVLLLVFSAFITLCFGAIFFLPDSSKLLSGVLFHSSPALQPAADHKPGPGARAEDAAEGRARRREEGAPGDPEAALEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKKKVAQDQLRDKAPFRGLPPVDFVPPIGVESREPADAAIREKRAKIKEMMKHAWNNYKGYAWGLNELKPISKGGHSSSLFGNIKGATIVDALDTLFIMEMKHEFEEAKSWVEENLDFNVNAEISVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSGIGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGNPIFAEKVMNIRTVLNKLEKPQGLYPNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHLIRKSSSGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADAAPEGMAQHYLELGAEIARTCHESYNRTFMKLGPEAFRFDGGVEAIATRQNEKYYILRPEVMETYMYMWRLTHDPKYRKWAWEAVEALENHCRVNGGYSGLRDVYLLHESYDDVQQSFFLAETLKYLYLIFSDDDLLPLEHWIFNSEAHLLPILPKDKKEVEIREE |
| 预测分子量 | 72,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAN1A1重组蛋白的3篇参考文献摘要：
1. **文献名称**：*"Glycosylation of recombinant therapeutic proteins in mammalian cell systems: Role of Golgi α-mannosidase I (MAN1A1)"*
**作者**：Hossler, P., et al.
**摘要**：研究探讨了哺乳动物细胞(如CHO和HEK293)中重组蛋白的糖基化修饰机制，重点分析了MAN1A1在高尔基体中对N-聚糖的α-1.2-甘露糖苷水解作用及其对重组蛋白质量的影响。
2. **文献名称**：*"Structural and functional analysis of Golgi α-mannosidase IA (MAN1A1) in glycan processing"*
**作者**：Hansen, J.E., et al.
**摘要**：通过X射线晶体学解析了MAN1A1的三维结构，揭示了其催化域的关键氨基酸残基如何参与底物识别和切割，为重组蛋白糖基化工程提供了结构基础。
3. **文献名称**：*"Role of MAN1A1 in cancer progression: Insights from recombinant protein overexpression models"*
**作者**：Valdez, B.C., et al.
**摘要**：通过体外重组MAN1A1蛋白过表达实验，发现其通过调控细胞表面糖蛋白的成熟，影响肿瘤细胞迁移和侵袭能力，提示其在癌症治疗中的潜在靶点价值。
4. **文献名称**：*"Recombinant MAN1A1 production in insect cells: Optimization and enzymatic characterization"*
**作者**：Zhang, Y., et al.
**摘要**：报道了利用杆状病毒-昆虫细胞系统高效表达重组MAN1A1蛋白的工艺优化，并验证了其体外催化活性，为大规模制备功能性酶提供参考。
(注：以上文献为示例，实际引用需以真实发表的论文为准。)
**Background of MAN1A1 Recombinant Protein**
MAN1A1 (Mannosidase Alpha Class 1A Member 1) is a Golgi-resident enzyme belonging to glycosyl hydrolase family 47. It catalyzes the trimming of mannose residues from N-linked glycans during protein maturation, a critical step in ensuring proper protein folding, quality control, and secretion. By sequentially removing α-1.2-linked mannose moieties, MAN1A1 facilitates the formation of complex glycans, which influence cell-cell interactions, immune recognition, and intracellular trafficking. Dysregulation of MAN1A1 has been linked to cancer metastasis, congenital disorders of glycosylation, and other diseases, highlighting its role in cellular homeostasis.
Recombinant MAN1A1 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional enzyme for research and therapeutic applications. This recombinant form retains catalytic activity and structural integrity, enabling studies on glycan-processing mechanisms, substrate specificity, and inhibitor screening. Its applications span structural biology (e.g., crystallography to resolve active-site architecture), cancer research (investigating metastasis-related glycosylation changes), and drug development (targeting glycosylation enzymes).
Additionally, recombinant MAN1A1 serves as a tool to explore endoplasmic reticulum-associated degradation (ERAD) pathways, where improper glycan trimming triggers misfolded protein disposal. Its availability accelerates biomarker discovery and personalized therapies, particularly in cancers with aberrant glycosylation profiles. Overall, MAN1A1 recombinant protein bridges fundamental glycobiology with translational research, offering insights into disease mechanisms and therapeutic opportunities.
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