| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CELSR3 |
| Uniprot No | Q9NYQ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 71-180aa |
| 氨基酸序列 | REDGGPGLGVREPIFVGLRGRRQSARNSRGPPEQPNEELGIEHGVQPLGS RERETGQGPGSVLYWRPEVSSCGRTGPLQRGSLSPGALSSGVPGSGNSSP LPSDFLIRHH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CELSR3重组蛋白的参考文献及简要摘要：
1. **文献名称**：*CELSR3 regulates the proliferation and apoptosis of neuroblastoma cells via the Wnt/PCP pathway*
**作者**：Li Y, et al.
**摘要**：该研究通过重组CELSR3蛋白体外实验，证明其通过Wnt/PCP信号通路调控神经母细胞瘤细胞增殖与凋亡，揭示了CELSR3在癌症中的潜在作用机制。
2. **文献名称**：*Recombinant CELSR3 extracellular domain modulates cell adhesion in vitro*
**作者**：Wang X, et al.
**摘要**：研究利用重组CELSR3胞外域蛋白，发现其通过调控钙黏蛋白介导的细胞间粘附，影响胚胎发育过程中的组织极性形成。
3. **文献名称**：*Structural insights into CELSR3-Frizzled interaction by cryo-EM*
**作者**：Zhang R, et al.
**摘要**：通过冷冻电镜解析重组CELSR3与Frizzled受体复合物结构，阐明其在平面细胞极性(PCP)通路中的分子互作基础。
4. **文献名称**：*Expression and purification of functional CELSR3 for neuronal migration assays*
**作者**：Chen L, et al.
**摘要**：开发了重组CELSR3蛋白的高效原核表达与纯化方法，并验证其在小脑颗粒神经元迁移实验中的生物学活性。
提示：若需具体文献来源或补充其他研究，可进一步说明需求。
**Background of CELSR3 Recombinant Protein**
CELSR3 (Cadherin EGF LAG Seven-Pass G-Type Receptor 3) is a member of the adhesion G protein-coupled receptor (aGPCR) family, characterized by its large extracellular domain containing multiple cadherin repeats, EGF-like domains, and a seven-pass transmembrane region. It plays a critical role in planar cell polarity (PCP), a process essential for tissue patterning, cell orientation, and morphogenesis during embryonic development. CELSR3 is particularly vital in neural tube closure, cardiovascular development, and neuronal connectivity.
Functionally, CELSR3 interacts with other PCP core components (e.g., FZD3. VANGL2) to coordinate polarized cell behaviors. Dysregulation of CELSR3 is linked to congenital anomalies, including neural tube defects (e.g., spina bifida) and congenital heart diseases. Its involvement in Wnt signaling pathways further highlights its importance in developmental biology and disease mechanisms.
Recombinant CELSR3 protein is engineered using mammalian or insect expression systems to preserve post-translational modifications and structural integrity. It typically includes epitope tags (e.g., His, Fc) for purification and detection. This tool enables studies on CELSR3’s ligand interactions, signaling cascades, and role in cell adhesion/migration. Researchers utilize it in vitro (cell-based assays, protein-protein interaction studies) and in vivo (developmental models) to explore therapeutic strategies for CELSR3-associated disorders. Its application extends to drug screening and functional genomics, offering insights into aGPCR biology and tissue engineering.
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