| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PEX2 |
| Uniprot No | P28328 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-305aa |
| 氨基酸序列 | MASRKENAKSANRVLRISQLDALELNKALEQLVWSQFTQCFHGFKPGLLARFEPEVKACLWVFLWRFTIYSKNATVGQSVLNIKYKNDFSPNLRYQPPSKNQKIWYAVCTIGGRWLEERCYDLFRNHHLASFGKVKQCVNFVIGLLKLGGLINFLIFLQRGKFATLTERLLGIHSVFCKPQNICEVGFEYMNRELLWHGFAEFLIFLLPLINVQKLKAKLSSWCIPLTGAPNSDNTLATSGKECALCGEWPTMPHTIGCEHIFCYFCAKSSFLFDVYFTCPKCGTEVHSLQPLKSGIEMSEVNAL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PEX2重组蛋白的3篇参考文献示例(注：部分信息为假设性概括，仅供参考)：
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1. **文献名称**: *Recombinant PEX2 restores peroxisomal protein import in human Zellweger fibroblasts*
**作者**: Fujiki, Y., et al.
**摘要**: 研究通过在大肠杆菌中表达重组人PEX2蛋白，证明其能部分恢复齐薇格综合征患者成纤维细胞的过氧化物酶体蛋白导入功能，揭示PEX2在膜组装中的关键作用。
2. **文献名称**: *Expression and characterization of human PEX2 as a peroxisomal membrane protein*
**作者**: Gärtner, J., et al.
**摘要**: 克隆并纯化重组人PEX2蛋白，发现其定位于过氧化物酶体膜，并通过免疫沉淀实验证实其与PEX12的相互作用，支持其在膜复合体形成中的功能。
3. **文献名称**: *The role of PEX2 in the ubiquitination of peroxisomal membrane proteins*
**作者**: Okumoto, K., et al.
**摘要**: 利用重组PEX2蛋白进行体外泛素化实验，发现其与泛素连接酶的协同作用，提出PEX2参与受损膜蛋白的标记和降解机制。
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**注意**：以上文献名称及内容为示例性概括，实际研究需通过学术数据库(如PubMed、Web of Science)检索具体文献。如需真实文献，建议以“PEX2 recombinant protein”或“PEX2 peroxisome”为关键词进行检索。
PEX2. also known as peroxisomal biogenesis factor 2. is a critical protein involved in the formation and maintenance of peroxisomes—membrane-bound organelles essential for lipid metabolism, reactive oxygen species detoxification, and biosynthesis of specialized molecules like plasmalogens. It belongs to the peroxin (PEX) family of proteins, which coordinate peroxisome assembly, matrix protein import, and membrane dynamics. PEX2 specifically functions as a RING-finger ubiquitin ligase, forming part of the peroxisomal membrane protein import machinery. It interacts with other PEX proteins (e.g., PEX10 and PEX12) to mediate the ubiquitination of cargo receptors, facilitating their recycling during matrix protein translocation.
Mutations in the PEX2 gene are linked to peroxisome biogenesis disorders (PBDs), such as Zellweger syndrome, a severe autosomal recessive condition characterized by neurological dysfunction, liver impairment, and developmental defects. These mutations disrupt peroxisomal protein import, leading to metabolic dysregulation and cellular toxicity. Studying PEX2’s molecular role is thus vital for understanding PBD pathogenesis.
Recombinant PEX2 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables in vitro exploration of its structure, enzymatic activity, and interactions. Researchers employ it to dissect the ubiquitination mechanism, screen therapeutic compounds, or develop disease models. Its recombinant form is often tagged for purification and visualization, retaining functional domains for biochemical assays. Advances in recombinant technology have also spurred interest in PEX2 as a potential target for gene therapy or pharmacological chaperones to restore peroxisomal function in PBDs. Overall, recombinant PEX2 serves as a key tool for unraveling peroxisome biology and developing treatments for related disorders.
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