| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C2CD3 |
| Uniprot No | Q4AC94 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于C2CD3重组蛋白的文献示例(注：部分文献为虚构示例，仅作格式参考)：
1. **文献名称**：C2CD3 is essential for centriole distal appendage assembly and ciliary signaling
**作者**：Smith J. et al.
**摘要**：研究发现C2CD3重组蛋白在纤毛形成中起关键作用，通过调控中心粒远端附属结构的组装，影响Hedgehog信号通路，突变会导致人类骨骼发育异常。
2. **文献名称**：Structural analysis of recombinant C2CD3 reveals its calcium-dependent membrane binding properties
**作者**：Lee S. et al.
**摘要**：通过重组表达纯化C2CD3蛋白，解析其C端结构域的钙依赖性膜结合机制，揭示了其在细胞膜定位和纤毛发生中的分子基础。
3. **文献名称**：C2CD3 mutations disrupt ciliogenesis and cause orofaciodigital syndrome in zebrafish models
**作者**：Garcia R. et al.
**摘要**：利用斑马鱼模型证明C2CD3基因突变导致纤毛缺陷，重组蛋白回补实验可恢复表型，为相关人类颅面发育综合征提供机制解释。
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**提示**：实际研究中建议通过PubMed或Google Scholar检索真实文献(关键词：C2CD3. recombinant protein, cilia, centriole)。该蛋白主要关联纤毛疾病及发育生物学领域。
C2CD3 (C2 calcium-dependent domain-containing protein 3) is a centrosome-associated protein critical for centriole elongation, ciliogenesis, and embryonic development. First identified through homology studies of C2 domain-containing proteins, it plays a key role in regulating the assembly and stability of primary cilia—a sensory organelle essential for signal transduction pathways like Hedgehog and Wnt. Structurally, C2CD3 contains an N-terminal C2 domain, which mediates calcium-dependent membrane interactions, and multiple coiled-coil regions for protein-protein interactions. Its localization to the distal end of centrioles enables recruitment of proteins required for ciliary membrane formation and microtubule anchoring.
Mutations in the C2CD3 gene are linked to human ciliopathies, including Jeune asphyxiating thoracic dystrophy (JATD) and short-rib polydactyly syndrome (SRPS), characterized by skeletal abnormalities and organ defects. Studies in model organisms, such as C2cd3-knockout mice, reveal embryonic lethality with severe craniofacial and heart malformations, underscoring its developmental importance.
Recombinant C2CD3 protein, typically produced in mammalian or insect expression systems, retains post-translational modifications necessary for functional studies. It is widely used to investigate ciliogenesis mechanisms, dissect disease-related mutations, and screen therapeutic compounds targeting ciliary dysfunction. Recent advances also explore its potential in gene therapy vectors to restore ciliary function in congenital disorders. As a research tool, recombinant C2CD3 bridges structural biology, developmental genetics, and translational medicine, offering insights into cilia-related cellular processes and therapeutic strategies.
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