Recombinant Human EMR1 protein

中文名： 含EGF样模块粘蛋白样激素受体1(EMR1)重组蛋白
别名： EMR1；EMR1；TM7LN3；Adhesion G protein-coupled receptor E1

货号: PA1000-8467

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	EMR1
Uniprot No	Q14246-2
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-821aa
氨基酸序列	MRGFNLLLFWGCCVMHSWEGHIRPTRKPNTKGNNCRDSTLCPAYATCTNT VDSYYCACKQGFLSSNGQNHFKDPGVRCKDIDECSQSPQPCGPNSSCKNL SGRYKCSCLDGFSSPTGNDWVPGKPGNFSCTDINECLTSSVCPEHSDCVN SMGSYSCSCQVGFISRNSTCEDVDECADPRACPEHATCNNTVGNYSCFCN PGFESSSGHLSFQGLKASCEDIDECTEMCPINSTCTNTPGSYFCTCHPGF APSNGQLNFTDQGVECRDIDECRQDPSTCGPNSICTNALGSYSCGCIAGF HPNPEGSQKDGNFSCQRVLFKCKEDVIPDNKQIQQCQEGTAVKPAYVSFC AQINNIFSVLDKVCENKTTVVSLKNTTESFVPVLKQISTWTKFTKEETSS LATVFLESVESMTLASFWKPSANITPAVRTEYLDIESKVINKECSEENVT LDLVAKGDKMKIGCSTIEESESTETTGVAFVSFVGMESVLNERFFKDHQA PLTTSEIKLKMNSRVVGGIMTGEKKDGFSDPIIYTLENIQPKQKFERPIC VSWSTDVKGGRWTSFGCVILEASETYTICSCNQMANLAIIMASGELTMGC AIIAGFLHYLFLACFFWMLVEAVILFLMVRNLKVVNYFSSRNIKMLHICA FGYGLPMLVVVISASVQPQGYGMHNRCWLNTETGFIWSFLGPVCTVIVIN SLLLTWTLWILRQRLSSVNAEVSTLKDTRLLTFKAFAQLFILGCSWVLGI FQIGPVAGVMAYLFTIINSLQGAFIFLIHCLLNGQVREEYKRWITGKTKP SSQSQTSRILLSSMPSASKTG
预测分子量	117 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于EMR1(ADGRE1/F4/80蛋白)重组蛋白研究的示例参考文献(内容为模拟概括，建议通过学术数据库验证具体文献)：

1. **文献名称**: "Characterization of the EMR1 (F4/80) recombinant protein in murine macrophage function"

**作者**: Smith A, et al.

**摘要**: 研究通过重组表达小鼠EMR1胞外域蛋白，分析其与配体(如CD37)的相互作用，发现其介导巨噬细胞黏附和炎症信号传递的功能。

2. **文献名称**: "Expression and purification of human EMR1 extracellular domain in mammalian cells"

**作者**: Lee JH, et al.

**摘要**: 报道了利用HEK293细胞系统重组表达人源EMR1胞外结构域的方法，并通过质谱验证其糖基化修饰，为结构生物学研究提供工具。

3. **文献名称**: "EMR1 as a biomarker for tumor-associated macrophages: Recombinant protein-based detection assay development"

**作者**: Wang X, et al.

**摘要**: 开发基于重组EMR1蛋白的抗体检测体系，验证其在肿瘤微环境中巨噬细胞特异性标记的应用潜力。

4. **文献名称**: "Structural insights into EMR1 ligand binding through recombinant chimeric protein analysis"

**作者**: Garcia R, et al.

**摘要**: 通过构建EMR1与IgG-Fc融合的重组蛋白，解析其与细胞表面配体结合的构效关系，揭示其参与免疫调控的分子机制。

**注意**：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science检索关键词(如"EMR1 recombinant protein" "ADGRE1 expression")获取真实文献。

背景信息

**Background of EMR1 Recombinant Protein**

EMR1 (EGF-like module-containing mucin-like hormone receptor-like 1), also known as ADGRE1. is a member of the adhesion G protein-coupled receptor (GPCR) family. It is characterized by a unique structure comprising an extracellular N-terminal region with multiple epidermal growth factor (EGF)-like domains, a mucin-like stalk, and a seven-transmembrane domain typical of GPCRs. EMR1 is predominantly expressed on immune cells, particularly tissue-resident macrophages, where it serves as a key surface marker. Its expression pattern has made it a valuable target for studying macrophage biology and immune regulation.

Functionally, EMR1 is implicated in cell adhesion, migration, and intercellular communication. Studies suggest its role in modulating inflammatory responses, tissue homeostasis, and phagocytosis. The receptor interacts with extracellular ligands, such as CD55. to regulate immune cell activation and signaling pathways. Dysregulation of EMR1 has been linked to pathological conditions, including chronic inflammation, fibrosis, and cancer, highlighting its therapeutic potential.

Recombinant EMR1 protein is engineered to facilitate *in vitro* and *in vivo* research. It retains functional domains necessary for ligand binding and signaling, enabling studies on receptor-ligand interactions, macrophage behavior, and immune modulation. This tool is critical for developing targeted therapies, such as monoclonal antibodies or small molecules, to modulate EMR1 activity in diseases involving aberrant macrophage function.

Overall, EMR1 recombinant protein serves as a vital resource for dissecting the receptor’s biological roles and advancing translational research in immunology and oncology.