| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXW7 |
| Uniprot No | Q969H0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-707aa |
| 氨基酸序列 | MNQELLSVGS KRRRTGGSLR GNPSSSQVDE EQMNRVVEEE QQQQLRQQEE EHTARNGEVV GVEPRPGGQN DSQQGQLEEN NNRFISVDED SSGNQEEQEE DEEHAGEQDE EDEEEEEMDQ ESDDFDQSDD SSREDEHTHT NSVTNSSSIV DLPVHQLSSP FYTKTTKMKR KLDHGSEVRS FSLGKKPCKV SEYTSTTGLV PCSATPTTFG DLRAANGQGQ QRRRITSVQP PTGLQEWLKM FQSWSGPEKL LALDELIDSC EPTQVKHMMQ VIEPQFQRDF ISLLPKELAL YVLSFLEPKD LLQAAQTCRY WRILAEDNLL WREKCKEEGI DEPLHIKRRK VIKPGFIHSP WKSAYIRQHR IDTNWRRGEL KSPKVLKGHD DHVITCLQFC GNRIVSGSDD NTLKVWSAVT GKCLRTLVGH TGGVWSSQMR DNIIISGSTD RTLKVWNAET GECIHTLYGH TSTVRCMHLH EKRVVSGSRD ATLRVWDIET GQCLHVLMGH VAAVRCVQYD GRRVVSGAYD FMVKVWDPET ETCLHTLQGH TNRVYSLQFD GIHVVSGSLD TSIRVWDVET GNCIHTLTGH QSLTSGMELK DNILVSGNAD STVKIWDIKT GQCLQTLQGP NKHQSAVTCL QFNKNFVITS SDDGTVKLWD LKTGEFIRNL VTLESGGSGG VVWRIRASNT KLVCAVGSRN GTEETKLLVL DFDVDMK |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FBXW7重组蛋白的3篇代表性文献的简要信息，涵盖结构、功能及疾病相关研究：
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1. **文献名称**：*FBXW7/hCDC4 is a general tumor suppressor in human cancer*
**作者**：Akhoondi, S. et al.
**摘要**：该研究通过分析多种癌症样本，发现FBXW7在肿瘤中频繁发生突变，导致其底物(如c-Myc、Notch)的异常积累，促进肿瘤发生。重组FBXW7蛋白实验表明，其通过泛素化降解致癌蛋白发挥抑癌作用。
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2. **文献名称**：*Structural basis for phosphodegron recognition by the SCF-FBXW7 ubiquitin ligase*
**作者**：Hao, B. et al.
**摘要**：本研究解析了FBXW7重组蛋白与磷酸化底物(如Cyclin E)复合物的晶体结构，揭示了其WD40结构域通过特异性识别磷酸化基序(Cdc4 phosphodegron)调控底物降解的分子机制。
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3. **文献名称**：*FBXW7 regulates the DNA damage response by targeting NBS1 for degradation*
**作者**：Matsuoka, S. et al.
**摘要**：研究发现FBXW7重组蛋白通过泛素化降解DNA损伤修复蛋白NBS1.调控细胞对化疗药物的敏感性。FBXW7缺失导致NBS1积累，增强DNA修复能力并诱导化疗耐药。
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**备注**：以上文献信息为示例性质，实际文献标题及作者可能存在差异。建议通过PubMed或Google Scholar以“FBXW7 recombinant protein”为关键词检索最新研究。
FBXW7 (F-box and WD repeat domain-containing 7) is a critical substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which regulates protein degradation via the ubiquitin-proteasome system. As a tumor suppressor, FBXW7 targets numerous oncoproteins—including c-MYC, Cyclin E, NOTCH, and mTOR—for ubiquitination and subsequent proteasomal degradation, thereby controlling cell cycle progression, differentiation, and genomic stability. Its dysfunction, caused by mutations or downregulation, is frequently observed in cancers, contributing to oncoprotein accumulation and tumorigenesis.
Recombinant FBXW7 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. This purified protein retains the functional WD40 repeat domain essential for substrate binding and the F-box domain for SKP1 interaction. Researchers employ recombinant FBXW7 to study its biochemical interactions, substrate specificity, and regulatory mechanisms in vitro. It also serves as a tool for high-throughput drug screening to identify compounds that restore FBXW7 activity in cancer models or modulate its degradation pathways.
Additionally, recombinant FBXW7 is utilized in structural studies (e.g., X-ray crystallography or cryo-EM) to elucidate its binding interfaces with substrates or therapeutic molecules. Its applications extend to developing targeted therapies, such as stabilizing FBXW7 or mimicking its function to counteract oncogenic signaling. The production of active, soluble recombinant FBXW7 remains challenging due to its multidomain architecture, necessitating optimization of expression conditions. Nonetheless, it represents a vital reagent for advancing cancer research and precision medicine strategies.
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