| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALU |
| Uniprot No | O43852 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-315aa |
| 氨基酸序列 | K PTEKKDRVHH EPQLSDKVHN DAQSFDYDHD AFLGAEEAKT FDQLTPEESK ERLGKIVSKI DGDKDGFVTV DELKDWIKFA QKRWIYEDVE RQWKGHDLNE DGLVSWEEYK NATYGYVLDD PDPDDGFNYK QMMVRDERRF KMADKDGDLI ATKEEFTAFL HPEEYDYMKD IVVQETMEDI DKNADGFIDL EEYIGDMYSH DGNTDEPEWV KTEREQFVEF RDKNRDGKMD KEETKDWILP SDYDHAEAEA RHLVYESDQN KDGKLTKEEI VDKYDLFVGS QATDFGEALV RHDEF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CALU(Calumenin)重组蛋白的示例文献摘要(注：文献信息为示例，实际引用请查询真实数据库)：
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1. **文献名称**: *"Recombinant Expression and Calcium-Binding Characterization of Human Calumenin"*
**作者**: Zhang L, et al.
**摘要**: 本研究在大肠杆菌系统中成功表达了带有His标签的重组人源Calumenin蛋白，通过亲和层析纯化后，利用荧光光谱法证实其与Ca²⁺的特异性结合能力，为后续研究其在细胞内钙调控机制提供基础。
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2. **文献名称**: *"Calumenin Inhibits Platelet Aggregation: Insights from Recombinant Protein Studies"*
**作者**: Tanaka K, et al.
**摘要**: 通过哺乳动物细胞表达系统获得功能性重组Calumenin，体外实验表明其通过结合血小板表面受体抑制凝血酶诱导的血小板聚集，提示CALU在心血管疾病中的潜在治疗价值。
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3. **文献名称**: *"Structural Analysis of Recombinant Calumenin Reveals ER Chaperone-like Activity"*
**作者**: Müller R, et al.
**摘要**: 采用昆虫细胞系统表达重组Calumenin，通过X射线晶体学解析其三维结构，发现其与内质网伴侣蛋白GRP78的相互作用，证明CALU参与错误折叠蛋白的修复过程。
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建议通过PubMed或Web of Science搜索关键词“recombinant Calumenin”或“CALU protein expression”获取真实文献。
**Background of CALU Recombinant Protein**
Calumenin (CALU), a member of the secretory pathway Ca²⁺-binding protein family, is a multifunctional protein encoded by the *CALU* gene in humans. It is characterized by six conserved EF-hand motifs, though it lacks strong calcium-binding capacity due to sequence variations. Primarily localized in the endoplasmic reticulum (ER) and Golgi apparatus, CALU plays roles in calcium homeostasis, protein folding, and post-translational modifications. It interacts with ER-resident chaperones like calreticulin and calnexin, contributing to quality control during glycoprotein synthesis.
CALU is implicated in diverse cellular processes, including cell adhesion, signaling, and apoptosis regulation. Studies highlight its involvement in cardiovascular diseases, cancer, and neurodegenerative disorders. For instance, CALU modulates ryanodine receptor (RyR) stability in cardiac cells, affecting calcium release and contractility. In cancer, aberrant CALU expression correlates with tumor progression, potentially influencing metastasis and drug resistance.
Recombinant CALU protein, produced via expression systems like *E. coli* or mammalian cells, retains functional properties for experimental applications. It serves as a tool to study protein-protein interactions, calcium signaling mechanisms, and pathological pathways. Researchers utilize it in assays to dissect its role in ER stress responses or to screen therapeutic agents targeting calcium dysregulation.
Overall, CALU recombinant protein is a critical reagent for unraveling the protein’s biological significance and translational potential in disease contexts. Its study bridges gaps between basic calcium signaling research and clinical applications.
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