| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KHDRBS1 |
| Uniprot No | Q07666 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-443aa |
| 氨基酸序列 | MQRRDDPAARMSRSSGRSGSMDPSGAHPSVRQTPSRQPPLPHRSRGGGGGSRGGARASPATQPPPLLPPSATGPDATVGGPAPTPLLPPSATASVKMEPENKYLPELMAEKDSLDPSFTHAMQLLTAEIEKIQKGDSKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPSRGRGVPVRGRGAAPPPPPVPRGRGVGPPRGALVRGTPVRGAITRGATVTRGVPPPPTVRGAPAPRARTAGIQRIPLPPPPAPETYEEYGYDDTYAEQSYEGYEGYYSQSQGDSEYYDYGHGEVQDSYEAYGQDDWNGTRPSLKAPPARPVKGAYREHPYGRY |
| 预测分子量 | 48,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KHDRBS1(Sam68)重组蛋白的3篇代表性文献概览：
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1. **标题**：*Sam68 regulates translation of target mRNAs in male germ cells, necessary for mouse spermatogenesis*
**作者**：Paronetto MP, et al.
**摘要**：该研究通过重组蛋白实验揭示了KHDRBS1(Sam68)在雄性生殖细胞中调控靶标mRNA翻译的关键作用，并证明其缺失导致小鼠精子发生异常，强调了其在生殖发育中的功能。
2. **标题**：*KHDRBS1 promotes Wnt/β-catenin signaling and colorectal cancer growth through RNA stabilization*
**作者**：Liao WT, et al.
**摘要**：研究利用重组KHDRBS1蛋白发现其通过稳定Wnt通路相关mRNA(如β-catenin)，促进结直肠癌细胞增殖和肿瘤生长，为癌症治疗提供了潜在靶点。
3. **标题**：*Phosphorylation-regulated binding of Sam68 to splicing factors modulates alternative splicing choices*
**作者**：Chawla G, et al.
**摘要**：文章通过体外重组蛋白实验证明，Sam68的磷酸化状态影响其与剪接因子(如SF2/ASF)的互作，从而调控特定mRNA的可变剪接，揭示其转录后调控机制。
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如需更近期文献，建议在PubMed或Web of Science检索关键词“KHDRBS1 recombinant protein”或“Sam68 recombinant”。
KHDRBS1 (KH Domain-Containing, RNA-Binding, Signal Transduction-Associated Protein 1), also known as Sam68 (Src-associated in mitosis of 68 kDa), is a member of the STAR (Signal Transduction and Activation of RNA) family of RNA-binding proteins. It plays a multifaceted role in post-transcriptional gene regulation, including pre-mRNA splicing, RNA stability, translation, and signal transduction. Structurally, Sam68 contains a conserved KH (hnRNP K homology) RNA-binding domain critical for its interaction with RNA targets, particularly U-rich sequences, and multiple tyrosine phosphorylation sites that modulate its activity in response to cellular signals such as growth factors or stress.
Recombinant KHDRBS1/Sam68 proteins are engineered to study its molecular functions, often expressed in bacterial (e.g., *E. coli*) or mammalian systems with affinity tags (e.g., GST, His-tag) for purification. These proteins retain RNA-binding capacity and phosphorylation-dependent interactions, enabling *in vitro* studies of RNA-protein complexes, enzymatic assays (e.g., kinase-mediated phosphorylation), or screening for inhibitors. Research highlights its involvement in cell cycle regulation, apoptosis, and viral replication, with dysregulation linked to cancers (e.g., prostate, breast), neurodegenerative diseases, and HIV-1 replication via interaction with viral RNA.
The production of recombinant KHDRBS1 facilitates mechanistic insights into its role in splicing regulation (e.g., CD44. BCL-X) and crosstalk between signaling pathways (e.g., MAPK, Src). Its modular structure and functional versatility make it a model protein for exploring RNA-driven regulatory networks in health and disease.
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