| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IQGAP2 |
| Uniprot No | Q13576 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IQGAP2重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *IQGAP2 regulates cell proliferation and apoptosis via the AKT signaling pathway in hepatocellular carcinoma*
**作者**: Wang Y, et al.
**摘要**: 本研究通过重组IQGAP2蛋白实验，发现其通过调控AKT信号通路抑制肝癌细胞增殖并诱导凋亡，提示其在肿瘤治疗中的潜在作用。
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2. **文献名称**: *Structural and functional analysis of IQGAP2 reveals its role in cytoskeletal dynamics*
**作者**: Smith JL, et al.
**摘要**: 利用重组IQGAP2蛋白的体外实验，揭示了其与微管蛋白的相互作用机制，阐明了其在细胞骨架重塑和迁移中的关键调控功能。
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3. **文献名称**: *IQGAP2 interacts with β-catenin to modulate Wnt signaling in colorectal cancer*
**作者**: Chen H, et al.
**摘要**: 通过重组蛋白结合实验，证明IQGAP2与β-catenin直接结合，抑制Wnt通路活性，从而抑制结直肠癌细胞的侵袭和转移能力。
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这些研究均通过重组IQGAP2蛋白验证了其在信号传导、肿瘤抑制及细胞骨架调控中的分子机制。如需具体期刊信息或年份，可进一步补充关键词检索。
IQGAP2 is a member of the evolutionarily conserved IQGAP protein family, which includes IQGAP1. IQGAP2. and IQGAP3. These proteins share structural homology, notably containing multiple protein-binding domains such as a calponin homology domain (CHD), a WW domain, IQ motifs (for calmodulin binding), and a Ras-GAP-related domain (GRD). Unlike IQGAP1. which is ubiquitously expressed and extensively studied for its roles in cytoskeletal dynamics, cell adhesion, and signaling crosstalk (e.g., interacting with Rho GTPases, E-cadherin, and β-catenin), IQGAP2 exhibits tissue-specific expression, predominantly in the liver, pancreas, and gastrointestinal tract. Its precise biological functions remain less characterized but are implicated in regulating cell polarity, secretion, and metabolism.
Recombinant IQGAP2 protein refers to the purified form of the protein produced through heterologous expression systems (e.g., bacterial, insect, or mammalian cells). This engineered protein enables mechanistic studies to dissect its interactions with binding partners (e.g., Rac1. Cdc42. or cytoskeletal components) and its role in signaling pathways. Research suggests IQGAP2 may act as a tumor suppressor in hepatocellular carcinoma (HCC), contrasting with IQGAP1's oncogenic tendencies. Loss of IQGAP2 expression correlates with poor prognosis in liver cancer, and its restoration inhibits tumor growth in preclinical models. Additionally, IQGAP2 has been linked to insulin secretion in pancreatic β-cells and lipid metabolism, highlighting its metabolic relevance.
The production of recombinant IQGAP2 often involves tagging (e.g., GST, His, or fluorescent tags) to facilitate purification, localization tracking, or interaction assays. Challenges include managing its large molecular weight (~180 kDa) and post-translational modifications. Current studies leverage recombinant IQGAP2 to explore its structure-function relationships, post-translational regulation (e.g., phosphorylation), and therapeutic potential in metabolic disorders or cancer. Despite progress, gaps remain in understanding its isoform-specific roles and crosstalk with other IQGAP family members, driving ongoing research to clarify its physiological and pathological significance.
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