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Recombinant Human EMR2 protein

  • 中文名: 含EGF样模块粘蛋白样激素受体2(EMR2)重组蛋白
  • 别    名: EMR2;EMR2;Adhesion G protein-coupled receptor E2
货号: PA1000-8466
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点EMR2
Uniprot No Q9UHX3
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间24-823aa
氨基酸序列QDSRGCARWCPQDSSCVNATACRCNPGFSSFSEIITTPMETCDDINECATLSKVSCGKFS DCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECQQNPRLCKSYGTCVNTLGSY TCQCLPGFKLKPEDPKLCTDVNECTSGQNPCHSSTHCLNNVGSYQCRCRPGWQPIPGSPN GPNNTVCEDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMT FSTWTPPPGVHSQTLSRFFDKVQDLGRDYKPGLANNTIQSILQALDELLEAPGDLETLPR LQQHCVASHLLDGLEDVLRGLSKNLSNGLLNFSYPAGTELSLEVQKQVDRSVTLRQNQAV MQLDWNQAQKSGDPGPSVVGLVSIPGMGKLLAEAPLVLEPEKQMLLHETHQGLLQDGSPI LLSDVISAFLSNNDTQNLSSPVTFTFSHRSVIPRQKVLCVFWEHGQNGCGHWATTGCSTI GTRDTSTICRCTHLSSFAVLMAHYDVQEEDPVLTVITYMGLSVSLLCLLLAALTFLLCKA IQNTSTSLHLQLSLCLFLAHLLFLVAIDQTGHKVLCSIIAGTLHYLYLATLTWMLLEALY LFLTARNLTVVNYSSINRFMKKLMFPVGYGVPAVTVAISAASRPHLYGTPSRCWLQPEKG FIWGFLGPVCAIFSVNLVLFLVTLWILKNRLSSLNSEVSTLRNTRMLAFKATAQLFILGC TWCLGILQVGPAARVMAYLFTIINSLQGVFIFLVYCLLSQQVREQYGKWSKGIRKLKTES EMHTLSSSAKADTSKPSTVN
预测分子量kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于EMR2重组蛋白的3篇参考文献及其摘要内容概括:

1. **文献名称**:*Structural characterization of the EMR2 extracellular domain and its interaction with chondroitin sulfate*

**作者**:Lin HH et al.

**摘要**:该研究通过重组表达EMR2的胞外结构域,解析其与硫酸软骨素的相互作用机制,揭示了EMR2在细胞黏附中的潜在功能。

2. **文献名称**:*EMR2 regulates neutrophil extracellular trap formation via PKC/MAPK signaling*

**作者**:Chen G et al.

**摘要**:利用重组EMR2蛋白进行功能实验,发现其通过激活PKC/MAPK通路调控中性粒细胞胞外诱捕网(NETs)的形成,提示其在炎症反应中的作用。

3. **文献名称**:*Recombinant EMR2-Fc融合蛋白抑制肿瘤细胞迁移的体外研究*

**作者**:王磊等

**摘要**:构建EMR2胞外区与人IgG Fc段的融合蛋白,证明其可通过竞争性结合微环境配体抑制肿瘤细胞迁移,为癌症治疗提供新策略。

注:以上文献为示例性内容,实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。若需具体文献,建议补充EMR2研究方向关键词(如功能、疾病关联等)。

背景信息

EMR2 (EGF-like module-containing mucin-like hormone receptor 2), also known as ADGRE2. is a member of the adhesion G protein-coupled receptor (ADGR or GPCR) subfamily. It is characterized by a large extracellular N-terminal region containing multiple epidermal growth factor (EGF)-like domains and a mucin-like stalk, followed by a seven-transmembrane domain typical of GPCRs. EMR2 is predominantly expressed on immune cells, including neutrophils, monocytes, and macrophages, where it plays roles in cell adhesion, migration, and immune response regulation. Its structure suggests involvement in ligand binding via extracellular domains, though its endogenous ligands remain under investigation.

The interest in EMR2 stems from its association with inflammatory diseases and cancer. For instance, dysregulated EMR2 expression has been linked to rheumatoid arthritis, atherosclerosis, and tumor progression, potentially through modulating leukocyte activation or tumor microenvironment interactions. To study its functions, recombinant EMR2 proteins are engineered using expression systems like mammalian or insect cells to ensure proper post-translational modifications (e.g., glycosylation). These recombinant proteins retain key functional domains and are used in ligand-binding assays, structural studies, and therapeutic target validation.

Recent research focuses on developing EMR2-targeted therapies, such as monoclonal antibodies or small-molecule inhibitors, to modulate immune responses or disrupt cancer pathways. Additionally, recombinant EMR2 serves as a tool to decipher signaling mechanisms, including its proposed cleavage-dependent activation, where proteolytic processing of the extracellular domain triggers receptor signaling. Overall, EMR2’s unique structure and immune-related roles make it a compelling subject for both basic research and translational applications.

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