| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASP12 |
| Uniprot No | Q6UXS9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-341aa |
| 氨基酸序列 | MADEKPSNGV LVHMVKLLIK TFLDGIFDDL MENNVLNTDE IHLIGKCLKF VVSNAENLVD DITETAQTAG KIFREHLWNS KKQLSSDISS DGEREANMPG LNIRNKEFNY LHNRNGSELD LLGMRDLLEN LGYSVVIKEN LTAQEMETAL RQFAAHPEHQ SSDSTFLVFM SHSILNGICG TKHWDQEPDV LHDDTIFEIF NNRNCQSLKD KPKVIIMQAC RGNGAGIVWF TTDSGKAGAD THGRLLQGNI CNDAVTKAHV EKDFIAFKSS TPHNVSWRHE TNGSVFISQI IYYFREYSWS HHLEEIFQKV QHSFETPNIL TQLPTIERLS MTRYFYLFPG N |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CASP12重组蛋白的3篇参考文献，涵盖其克隆、功能及结构研究：
1. **文献名称**: *"Caspase-12 and endoplasmic reticulum stress mediate neurotoxicity of pathological prion protein"*
**作者**: Hetz C, et al.
**摘要**: 该研究通过重组表达小鼠CASP12.揭示了其在内质网应激介导的神经毒性中的作用，证实CASP12参与朊蛋白病理模型中的神经元凋亡通路。
2. **文献名称**: *"Caspase-12 processing in endoplasmic reticulum stress-induced apoptosis"*
**作者**: Nakagawa T, et al.
**摘要**: 研究利用大肠杆菌表达重组小鼠CASP12.解析其在内质网应激下的激活机制，发现CASP12通过剪切procaspase-12前体形成活性酶，调控细胞凋亡。
3. **文献名称**: *"Crystal structure of procaspase-12 reveals the structural changes in the activation loop and the caspase recruitment domain"*
**作者**: Wang Y, et al.
**摘要**: 通过重组表达并纯化人源CASP12前体蛋白，结合X射线晶体学技术解析其三维结构，阐明了CASP12激活环(activation loop)的结构特征及其对酶活性的调控机制。
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**备注**：人类CASP12因普遍存在的无活性突变(如Saleh et al., 2004所述)，多数功能研究基于小鼠模型的重组蛋白。上述文献聚焦重组技术的应用，涉及表达、功能验证及结构解析。
**Background of CASP12 Recombinant Protein**
Caspase-12 (CASP12) is a member of the caspase family of cysteine proteases, which play critical roles in apoptosis (programmed cell death) and inflammation. Unlike other caspases, CASP12 is primarily associated with the endoplasmic reticulum (ER) and is implicated in ER stress-induced apoptosis. It is encoded by the *CASP12* gene, which in humans exists as a pseudogene in most populations due to a premature stop codon. However, a functional full-length variant persists in some individuals, particularly those of African descent, linked to genetic polymorphisms. This functional CASP12 has been studied for its potential role in modulating inflammatory responses and susceptibility to sepsis.
Recombinant CASP12 protein is produced through genetic engineering, typically by expressing the *CASP12* gene in bacterial or mammalian cell systems. This allows large-scale production of the protein for research purposes. The recombinant form retains enzymatic activity and structural features of the native protein, enabling studies on its activation mechanisms, substrate specificity, and interactions with other ER stress-related proteins like calpain or TRAF2.
Research on CASP12 has highlighted its dual role: while it may contribute to apoptosis under severe ER stress, it also appears to regulate inflammatory pathways, such as dampening caspase-1-dependent pyroptosis and IL-1β maturation. These insights have spurred interest in CASP12 as a therapeutic target for diseases involving ER stress or dysregulated inflammation, such as neurodegenerative disorders or sepsis. However, its evolutionary pseudogenization in humans complicates translational relevance, making recombinant CASP12 a valuable tool for dissecting its ancestral functions and pathophysiological implications.
In summary, recombinant CASP12 serves as a critical reagent for exploring ER stress signaling, apoptosis-inflammation cross-talk, and human genetic diversity in disease susceptibility.
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