| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASP4 |
| Uniprot No | P49662 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-377aa |
| 氨基酸序列 | MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKKYYDAKTEDKVRVMADSMQEKQRMAGQMLLQTFFNIDQISPNKKAHPNMEAGPPESGESTDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRNGADFDITGMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSHGILEGICGTVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGELWVRDSPASLEVASSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDSTMGSIFITQLITCFQKYSWCCHLEEVFRKVQQSFETPRAKAQMPTIERLSMTRYFYLFPGN |
| 预测分子量 | 45.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CASP4(Caspase-4)重组蛋白研究的3篇代表性文献，按研究主题简要概括：
1. **文献名称**：*Caspase-4 mediates non-canonical activation of the NLRP3 inflammasome in human myeloid cells*
**作者**：Shi J, Zhao Y, et al.
**摘要**：该研究阐明了CASP4在人类髓系细胞中通过非经典途径激活NLRP3炎症小体的机制，利用重组CASP4蛋白实验验证其直接结合脂多糖(LPS)并启动炎症反应。
2. **文献名称**：*Non-canonical inflammasome activation targets caspase-11*
**作者**：Kayagaki N, et al.
**摘要**：研究聚焦于CASP4(小鼠同源物为Caspase-11)的重组蛋白功能，证明其通过识别胞内革兰氏阴性菌脂多糖(LPS)触发细胞焦亡和炎症反应。
3. **文献名称**：*Caspase-4 exacerbates enteropathogenic E. coli infection through inflammasome activation*
**作者**：Knodler LA, et al.
**摘要**：通过重组CASP4蛋白实验，揭示其在肠道致病性大肠杆菌感染中通过炎症小体通路加剧宿主炎症反应的分子机制。
**备注**：CASP4在人类中的功能常与小鼠Caspase-11类比，部分文献可能以“Caspase-4/11”并称。以上文献可通过PubMed或Google Scholar搜索标题获取全文。
CASP4 (Caspase-4), also known as Caspase-11 in mice, is a member of the cysteine-aspartic protease (caspase) family, which plays critical roles in apoptosis, inflammation, and immune responses. As an inflammatory caspase, it is primarily involved in non-apoptotic pathways, particularly in the innate immune system. Structurally, CASP4 contains a caspase recruitment domain (CARD) at its N-terminus, followed by a catalytic domain comprising large (p20) and small (p10) subunits. Unlike apoptotic caspases, CASP4 is activated through proximity-induced autocatalysis upon recruitment to inflammasome complexes, such as those triggered by cytosolic lipopolysaccharide (LPS) or other pathogen-associated molecular patterns (PAMPs).
Recombinant CASP4 protein is engineered for in vitro studies to dissect its role in pyroptosis, a form of programmed cell death linked to inflammatory responses. Upon activation, CASP4 cleaves gasdermin D (GSDMD), generating pores in the cell membrane that lead to cytokine release and cell death. It also interacts with NLRP3 inflammasomes, amplifying inflammatory signaling. Recombinant CASP4 is typically produced in bacterial (e.g., E. coli) or mammalian expression systems, followed by purification via affinity tags (e.g., His-tag) and functional validation using substrate cleavage assays.
Research on recombinant CASP4 has advanced understanding of sepsis, neurodegenerative diseases, and autoimmune disorders, where dysregulated inflammasome activity is implicated. It is also used to screen caspase-specific inhibitors for therapeutic development. However, its murine homolog Caspase-11 often complicates cross-species studies, necessitating careful model selection. Overall, recombinant CASP4 serves as a vital tool for exploring inflammasome mechanisms and designing targeted anti-inflammatory therapies.
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