| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASP8AP2 |
| Uniprot No | Q9UKL3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **CASP8AP2(FLASH)重组蛋白** 的3篇参考文献及其简要摘要：
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1. **文献名称**：*FLASH is essential for caspase-8 activation in embryonic development and signal transduction*
**作者**：Yamaguchi Y et al.
**摘要**：研究通过重组CASP8AP2/FLASH蛋白，揭示其在胚胎发育中通过调控caspase-8激活途径促进凋亡的功能，并证实其与死亡诱导信号复合物(DISC)的相互作用。
2. **文献名称**：*Regulation of histone mRNA metabolism by FLASH/CASP8AP2*
**作者**：Yang XC et al.
**摘要**：利用重组FLASH蛋白进行体外实验，证明其通过结合组蛋白mRNA 3'末端加工复合体，调控组蛋白前体mRNA的剪切和稳定性，影响细胞周期进程。
3. **文献名称**：*Structural and functional analysis of the FLASH protein in transcriptional regulation*
**作者**：Szymczyk J et al.
**摘要**：通过重组CASP8AP2蛋白的晶体结构解析，揭示其N端结构域在招募转录因子(如c-Myc)中的作用，并验证其在癌症中异常表达对基因转录的影响。
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**备注**：若需具体文献链接或补充其他研究，可进一步提供关键词或数据库检索建议。
**Background of CASP8AP2 Recombinant Protein**
CASP8AP2 (Caspase 8-associated protein 2), also known as FLASH (FLICE-associated huge protein), is a multifunctional protein implicated in apoptosis, transcriptional regulation, and RNA processing. Initially identified as a binding partner of caspase-8. it plays a critical role in death receptor-mediated apoptosis by facilitating the assembly of the death-inducing signaling complex (DISC). This interaction promotes caspase-8 activation, a key step in extrinsic apoptotic signaling.
Beyond apoptosis, CASP8AP2 is involved in transcription elongation through its association with the positive transcription elongation factor b (P-TEFb), regulating RNA polymerase II activity. It also participates in histone mRNA processing by interacting with the 3′ end processing machinery, linking transcription and RNA maturation.
The recombinant CASP8AP2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Purified recombinant CASP8AP2 allows researchers to investigate its biochemical properties, structural interactions, and mechanisms in apoptosis and transcription. It serves as a tool for studying diseases linked to apoptotic dysregulation, such as cancer and autoimmune disorders, and may aid in therapeutic screening targeting caspase-8 pathways.
Studies using recombinant CASP8AP2 have clarified its dual roles in cell survival and death, highlighting its potential as a biomarker or therapeutic target. However, its precise molecular mechanisms and context-dependent functions remain under investigation, emphasizing the need for further research to unravel its complex regulatory networks.
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