| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARD9 |
| Uniprot No | Q9H257 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-536aa |
| 氨基酸序列 | MSDYENDDEC WSVLEGFRVT LTSVIDPSRI TPYLRQCKVL NPDDEEQVLS DPNLVIRKRK VGVLLDILQR TGHKGYVAFL ESLELYYPQL YKKVTGKEPA RVFSMIIDAS GESGLTQLLM TEVMKLQKKV QDLTALLSSK DDFIKELRVK DSLLRKHQER VQRLKEECEA GSRELKRCKE ENYDLAMRLA HQSEEKGAAL MRNRDLQLEI DQLKHSLMKA EDDCKVERKH TLKLRHAMEQ RPSQELLWEL QQEKALLQAR VQELEASVQE GKLDRSSPYI QVLEEDWRQA LRDHQEQANT IFSLRKDLRQ GEARRLRCME EKEMFELQCL ALRKDSKMYK DRIEAILLQM EEVAIERDQA IATREELHAQ HARGLQEKDA LRKQVRELGE KADELQLQVF QCEAQLLAVE GRLRRQQLET LVLSSDLEDG SPRRSQELSL PQDLEDTQLS DKGCLAGGGS PKQPFAALHQ EQVLRNPHDA GLSSGEPPEK ERRRLKESFE NYRRKRALRK MQKGWRQGEE DRENTTGSDN TDTEGS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"CARD9 Signaling in Innate Immunity and Inflammation"** by Gringhuis, S.I. et al.
*摘要：探讨CARD9重组蛋白在介导抗真菌免疫反应中的作用，解析其通过NF-κB通路激活炎症因子的机制。*
2. **"Structural Basis of CARD9-BCL10 Complex Assembly in Innate Immunity"** by Jiang, J. et al.
*摘要：利用重组CARD9蛋白进行结构解析，揭示其与BCL10相互作用的关键结构域，阐明先天免疫信号传导的分子基础。*
3. **"CARD9 Deficiency and Fungal Infection Susceptibility"** by Wang, X. et al.
*摘要：通过体外重组CARD9蛋白功能实验，验证患者基因突变导致的免疫缺陷，证明CARD9在宿主抗真菌防御中的必要性。*
4. **"Recombinant CARD9 Protein Ameliorates Colitis in Murine Models"** by Liang, Q. et al.
*摘要：研究重组CARD9蛋白对肠道炎症的调控作用，发现其通过修复免疫细胞信号通路缓解实验性结肠炎。*
(注：以上文献信息为示例，实际引用需核实具体期刊及年份。)
**Background of CARD9 Recombinant Protein**
CARD9 (Caspase Recruitment Domain-containing protein 9) is an adaptor protein critical for immune signaling, particularly in innate immunity. It belongs to the CARD protein family, which mediates interactions between caspases, kinases, and other signaling molecules via homotypic CARD-CARD domain binding. CARD9 acts downstream of pattern recognition receptors (PRRs), such as C-type lectin receptors (CLRs), which detect fungal and bacterial pathogens. Upon activation, CARD9 forms a complex with BCL10 and MALT1. triggering NF-κB and MAPK signaling pathways to promote pro-inflammatory cytokine production and immune cell activation.
CARD9 is highly expressed in myeloid cells (e.g., macrophages, dendritic cells) and plays a pivotal role in antifungal immunity. Genetic mutations in *CARD9* are linked to increased susceptibility to fungal infections (e.g., chronic mucocutaneous candidiasis) and autoimmune or inflammatory disorders like inflammatory bowel disease (IBD). These mutations often impair immune cell recruitment, pathogen recognition, or downstream signaling, highlighting its non-redundant function in host defense.
Recombinant CARD9 protein is engineered for in vitro and in vivo studies to dissect its molecular mechanisms, interactions, and therapeutic potential. Produced via bacterial or mammalian expression systems, it retains functional domains for binding partners (e.g., BCL10) and can be used in assays such as pull-down experiments, kinase activity tests, or cell-based signaling studies. Researchers also utilize recombinant CARD9 to model pathogenic mutations, screen drug candidates targeting CARD9-associated pathways, or develop diagnostic tools for immune dysregulation syndromes.
Overall, CARD9 recombinant protein serves as a vital tool for unraveling immune signaling networks and advancing therapies for infections and immune-mediated diseases.
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