| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CETN1 |
| Uniprot No | Q12798 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MASGFKKPSAASTGQKRKVAPKPELTEDQKQEVREAFDLFDVDGSGTIDAKELKVAMRALGFEPRKEEMKKMISEVDREGTGKISFNDFLAVMTQKMSEKDTKEEILKAFRLFDDDETGKISFKNLKRVANELGENLTDEELQEMIDEADRDGDGEVNEEEFLRIMKKTSLY |
| 预测分子量 | 46.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CETN1重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献请通过学术数据库查询)：
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1. **标题**: *Efficient Expression and Purification of Recombinant Human CETN1 in E. coli*
**作者**: Zhang L, Li H, et al.
**摘要**: 本研究成功构建了CETN1的重组质粒，通过优化大肠杆菌表达条件实现高效可溶性表达，并利用镍柱亲和层析纯化获得高纯度蛋白。圆二色谱分析证实其二级结构与天然蛋白一致，为功能研究提供可靠材料。
2. **标题**: *Structural Insights into Calcium Binding of Recombinant CETN1*
**作者**: Martinez R, Kim S, et al.
**摘要**: 通过X射线晶体学解析了重组CETN1的钙离子结合结构域，揭示了其EF-hand基序的构象变化，阐明了钙信号调控CETN1参与中心体组装和细胞分裂的分子机制。
3. **标题**: *CETN1 Recombinant Protein Interacts with XPC in DNA Damage Repair*
**作者**: Sugasawa K, Ng JM, et al.
**摘要**: 体外实验表明，重组CETN1蛋白与XPC形成复合物，增强其对UV诱导DNA损伤的识别能力，证实CETN1在核苷酸切除修复(NER)通路中的关键辅助作用。
4. **标题**: *Functional Characterization of CETN1 in Cancer Cell Lines Using Recombinant Protein*
**作者**: Patel S, Gupta R, et al.
**摘要**: 通过外源添加重组CETN1蛋白，发现其可抑制肝癌细胞HepG2的迁移和侵袭，并下调MMP-9表达，提示CETN1可能作为肿瘤治疗的潜在靶点。
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**提示**：实际研究中，建议通过PubMed或Google Scholar以关键词“CETN1 recombinant protein”、“Centrin-1 expression”等检索最新文献，重点关注蛋白表达策略、结构功能研究及疾病相关机制等领域。
**Background of CETN1 Recombinant Protein**
Centrin-1 (CETN1) is a small, calcium-binding protein belonging to the EF-hand superfamily, highly conserved across eukaryotes. It plays a critical role in microtubule organization, centriole duplication, and cilia formation by regulating centrosome structure and function. CETN1 is closely associated with the centrosome, a key organelle involved in cell division, and interacts with other centrin family members (e.g., CETN2. CETN3) to maintain genomic stability.
Recombinant CETN1 protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems, followed by purification via affinity chromatography (e.g., His-tag). Its production enables detailed biochemical and structural studies, such as elucidating calcium-dependent conformational changes or binding partners like XPC (xeroderma pigmentosum group C) in DNA repair mechanisms.
Research on CETN1 has implications for understanding diseases linked to centrosomal dysfunction, including ciliopathies, microcephaly, and cancer. Overexpression or mutations in CETN1 may disrupt cell cycle regulation, contributing to tumorigenesis. Additionally, CETN1’s role in ciliary assembly highlights its relevance in sensory disorders and developmental abnormalities.
The recombinant protein also serves as a tool for antibody development, diagnostic assays, and high-throughput screening for therapeutic agents targeting centrosome-related pathologies. Despite its small size (~19 kDa), CETN1’s multifunctional nature underscores its importance in cellular homeostasis, making it a focal point in both basic and applied biomedical research.
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