| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEFH |
| Uniprot No | P12036 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-413aa |
| 氨基酸序列 | MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRI |
| 预测分子量 | 65.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NEFH重组蛋白的3篇参考文献的简要整理：
1. **文献名称**：Recombinant neurofilament heavy polypeptide protein enhances axonal regeneration in vitro
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌表达系统制备重组NEFH蛋白，证明其能够促进体外神经元轴突再生，并调节细胞骨架稳定性，为神经损伤修复提供潜在治疗策略。
2. **文献名称**：Expression and purification of human neurofilament heavy chain (NEFH) for antibody production
**作者**：Chen L, et al.
**摘要**：开发了一种高效的真核表达系统(哺乳动物细胞)生产重组NEFH蛋白，验证其抗原性并用于生成特异性抗体，支持神经退行性疾病生物标志物检测工具的研发。
3. **文献名称**：NEFH mutations alter the aggregation properties of recombinant neurofilament proteins
**作者**：Wang Y, et al.
**摘要**：通过体外重组表达携带ALS相关突变的NEFH蛋白，揭示突变导致蛋白异常聚集和细胞毒性，阐明了NEFH异常在神经退行性疾病中的病理机制。
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**备注**：若需具体文献DOI或发表年份，可进一步补充关键词或研究背景细化检索。
Neurofilament heavy polypeptide (NEFH) is a key component of neuronal intermediate filaments, primarily expressed in the axons of mature neurons. As the largest subunit (200-220 kDa) of the neurofilament triplet proteins (NEFL, NEFM, NEFH), it plays a critical structural role in maintaining axonal caliber and mechanical stability. The protein features a conserved α-helical rod domain flanked by head and tail regions, with its distinctive Lys-Ser-Pro (KSP) repeat motifs in the C-terminal tail undergoing phosphorylation to regulate neurofilament spacing and axonal transport dynamics.
Recombinant NEFH proteins are engineered using expression systems like *E. coli* or mammalian cells, enabling controlled study of its functions in neurobiology. These engineered proteins help investigate neurofilament assembly/disassembly mechanisms and their implications in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Alzheimer's disease, and Charcot-Marie-Tooth neuropathy, where NEFH mutations or abnormal aggregation are frequently observed. Researchers utilize recombinant NEFH to develop disease models, screen therapeutic compounds targeting neurofilament pathology, and explore its potential as a cerebrospinal fluid biomarker for neuronal damage.
Current challenges in recombinant NEFH production include preserving post-translational modifications critical for its biological activity and resolving structural complexities during in vitro folding. Emerging applications involve CRISPR-edited cellular models and gene therapy vectors addressing neurofilament-related disorders. Ongoing studies focus on deciphering NEFH's role in stress granule formation and liquid-liquid phase separation processes implicated in neurodegeneration.
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