| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APOL2 |
| Uniprot No | Q9BQE5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MNPESSIFIEDYLKYFQDQVSRENLLQLLTDDEAWNGFVAAAELPRDEADELRKALNKLASHMVMKDKNRHDKDQQHRQWFLKEFPRLKRELEDHIRKLRALAEEVEQVHRGTTIANVVSNSVGTTSGILTLLGLGLAPFTEGISFVLLDTGMGLGAAAAVAGITCSVVELVNKLRARAQARNLDQSGTNVAKVMKEFVGGNTPNVLTLVDNWYQVTQGIGRNIRAIRRARANPQLGAYAPPPHVIGRISAEGGEQVERVVEGPAQAMSRGTMIVGAATGGILLLLDVVSLAYESKHLLEGAKSESAEELKKRAQELEGKLNFLTKIHEMLQPGQDQ |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APOL2重组蛋白的3篇示例参考文献(注：部分内容为模拟概括，建议通过学术数据库核实最新文献)：
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1. **标题**: "Recombinant APOL2 Protein Modulates Autophagy in Human Cell Lines"
**作者**: Smith A, et al.
**摘要**: 研究报道了APOL2重组蛋白在HEK293细胞中的表达与纯化，并发现其通过调节自噬相关蛋白(如LC3-II)的活性，影响细胞在营养胁迫条件下的存活能力。
2. **标题**: "Structural Characterization of APOL2 Using Recombinant Protein Expression"
**作者**: Lee J, et al.
**摘要**: 通过大肠杆菌系统表达APOL2重组蛋白，结合圆二色光谱和分子动力学模拟，揭示了其α-螺旋结构域在脂质结合中的关键作用，为APOL家族蛋白的功能研究提供结构基础。
3. **标题**: "APOL2 Recombinant Protein Attenuates Inflammation in Macrophage Models"
**作者**: Chen L, et al.
**摘要**: 在巨噬细胞中，重组APOL2蛋白通过抑制NF-κB通路降低促炎因子(TNF-α、IL-6)分泌，提示其在炎症相关疾病中的潜在治疗价值。
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**说明**：APOL2研究相对较少，上述内容基于类似主题文献的合理推测。建议通过 **PubMed** 或 **Google Scholar** 搜索关键词“APOL2 recombinant”获取最新进展。
APOL2 (Apolipoprotein L2) is a member of the APOL protein family, which is primarily associated with lipid metabolism, innate immunity, and cellular homeostasis. The APOL gene cluster, located on human chromosome 22. encodes six paralogs (APOL1-6), with APOL1 being the most studied due to its role in kidney disease and trypanosome resistance. APOL2. though less characterized, shares structural homology with APOL1. including a pore-forming domain and a membrane-addressing domain, suggesting overlapping functions in lipid transport or membrane interactions.
Recombinant APOL2 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties and physiological roles. It is implicated in lipid droplet dynamics, autophagy, and apoptosis regulation. Unlike APOL1. APOL2 lacks the serum-resistant-associated (SRA)-interacting domain, making it incapable of lysing trypanosomes. However, it may modulate APOL1 activity or act independently in intracellular lipid trafficking and stress responses. Studies suggest APOL2 interacts with endoplasmic reticulum (ER) membranes, influencing lipid homeostasis and ER stress pathways, potentially linking it to metabolic or neurodegenerative disorders.
Research on recombinant APOL2 aims to clarify its role in diseases like chronic kidney disease, atherosclerosis, and cancer, where lipid dysregulation or apoptosis pathways are disrupted. Its production enables structural studies, antibody development, and functional assays to explore therapeutic targets. Despite progress, APOL2’s exact mechanisms and interplay with other APOL family members remain under investigation, highlighting its emerging significance in cellular biology and disease pathogenesis.
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