| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APOC1 |
| Uniprot No | P02654 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-83aa |
| 氨基酸序列 | TPDVSSALDKLKEFGNTLEDKARELISRIKQSELSAKMREWFSETFQKVKEKLKIDS |
| 预测分子量 | 22.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APOC1重组蛋白的3篇示例参考文献(内容为虚构，供参考)：
1. **《重组APOC1蛋白在神经炎症中的调控作用》**
*作者：Smith J, et al.*
摘要：本研究通过体外表达重组APOC1蛋白，探究其对小胶质细胞炎症反应的调控机制，发现APOC1通过抑制TLR4/NF-κB信号通路减轻神经炎症，为阿尔茨海默病治疗提供潜在靶点。
2. **《APOC1重组蛋白的制备及其与脂代谢的关联分析》**
*作者：Wang L, et al.*
摘要：利用大肠杆菌系统高效表达并纯化重组APOC1蛋白，证实其能够结合低密度脂蛋白(LDL)，调节胆固醇代谢，揭示其在动脉粥样硬化中的病理作用。
3. **《APOC1重组蛋白对Aβ沉积的体外影响研究》**
*作者：Chen Y, et al.*
摘要：通过构建APOC1重组蛋白与β淀粉样蛋白(Aβ)共培养模型，发现APOC1可促进Aβ聚集，提示其在阿尔茨海默病病程中可能加剧神经元损伤。
**提示**：以上文献为示例，实际文献需通过PubMed或Google Scholar搜索关键词“APOC1 recombinant protein”“APOC1 expression”获取。真实研究多聚焦于APOC1在脂代谢、神经退行性疾病及炎症中的功能机制。
Apolipoprotein C-I (APOC1) is a small, exchangeable apolipoprotein primarily synthesized in the liver and intestine. It plays a critical role in lipid metabolism by associating with triglyceride-rich lipoproteins (e.g., VLDL) and HDL, modulating their interactions with enzymes and receptors. APOC1 inhibits lipoprotein lipase (LPL), delaying triglyceride hydrolysis and prolonging lipoprotein circulation, and affects cholesterol homeostasis by influencing LCAT activity and HDL remodeling. It also binds the LDL receptor-related protein, potentially impacting lipid clearance.
APOC1 is linked to cardiovascular diseases due to its role in dyslipidemia and atherosclerosis. Elevated levels correlate with metabolic syndrome and Alzheimer’s disease, where it may promote amyloid-beta aggregation. Its gene resides within the APOE/APOC1/APOC2 cluster on chromosome 19. with polymorphisms influencing disease risk.
Recombinant APOC1 is produced via bacterial (e.g., E. coli) or eukaryotic expression systems, enabling studies on its structure-function relationships. Engineered variants often include tags (e.g., His-tag) for purification. Researchers use recombinant APOC1 to investigate lipid-protein interactions, inflammatory pathways, and therapeutic targets. Its small size (6.6 kDa) and solubility challenges necessitate optimized expression protocols. Studies highlight its dual role in lipid metabolism and neuropathology, making it a focus for drug development and biomarker research.
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