| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEO1 |
| Uniprot No | Q92859 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 160 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NEO1(Neogenin-1)重组蛋白的参考文献示例(内容为模拟概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *Structural insights into Neogenin-1 extracellular domain and its interaction with RGMa*
**作者**: Smith, J. et al.
**摘要**: 本研究通过重组表达NEO1的胞外结构域，利用X射线晶体学解析其三维结构，揭示了其与排斥性引导分子RGMa结合的分子机制，为神经损伤修复研究提供了结构基础。
2. **文献名称**: *Neogenin-1 regulates apoptosis in cancer cells via recombinant protein-mediated pathway analysis*
**作者**: Lee, H. & Zhang, Y.
**摘要**: 通过重组NEO1蛋白的体外实验，证明其与DCC(Deleted in Colorectal Cancer)蛋白的相互作用可激活caspase依赖的凋亡通路，提示其在肿瘤抑制中的潜在作用。
3. **文献名称**: *Recombinant Neogenin-1 promotes axon guidance in developing neurons*
**作者**: Thompson, R. et al.
**摘要**: 利用重组NEO1蛋白探究其对Netrin-1的响应机制，发现其在神经元轴突导向中通过Rho GTPase信号通路调控细胞骨架重排，支持其在神经发育中的功能。
4. **文献名称**: *Expression and purification of functional Neogenin-1 ectodomain for ligand screening*
**作者**: Kumar, S. et al.
**摘要**: 报道了一种高效重组表达和纯化NEO1胞外域的方法，验证其与配体结合活性，为高通量药物筛选提供了技术基础。
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建议通过PubMed、Web of Science等平台以关键词“Neogenin-1 recombinant protein”或“NEO1 ectodomain”检索最新文献。
Neogenin-1 (NEO1), a transmembrane receptor belonging to the immunoglobulin (Ig) superfamily, plays critical roles in developmental and pathological processes. Initially identified as a homolog of the tumor suppressor DCC (Deleted in Colorectal Cancer), NEO1 shares structural features including multiple Ig-like domains and fibronectin type III (FNIII) repeats in its extracellular region. It interacts with diverse ligands, such as netrins (guidance cues for axon growth), repulsive guidance molecules (RGMs), and bone morphogenetic proteins (BMPs), mediating cell-cell communication and signal transduction.
NEO1 is implicated in neural development, angiogenesis, tissue homeostasis, and cancer progression. During embryogenesis, it regulates axon guidance, neural tube closure, and neuronal survival. In adults, NEO1 modulates cell adhesion, apoptosis, and inflammatory responses. Dysregulation of NEO1 signaling has been linked to neurodevelopmental disorders, metastatic cancers, and ischemic injuries. For instance, its interaction with RGMs influences iron metabolism and neuronal death in neurodegenerative conditions, while its role in BMP signaling contributes to tumor suppression or progression depending on cellular context.
Recombinant NEO1 proteins, typically produced in mammalian or insect expression systems, retain functional extracellular domains for ligand-binding studies. These engineered proteins enable researchers to dissect NEO1’s structure-function relationships, screen therapeutic agents, and explore its role in disease mechanisms. Soluble NEO1-Fc fusion proteins, for example, are widely used to block ligand-receptor interactions in vitro and in vivo. Recent studies also highlight its potential as a biomarker or therapeutic target in cancer immunotherapy and neural repair. By leveraging recombinant NEO1 tools, ongoing research aims to unravel its dual roles in homeostasis and pathology, offering insights into targeted interventions for neurological and oncological diseases.
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