| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MUC20 |
| Uniprot No | Q8N307 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-709aa |
| 氨基酸序列 | GPSTR RADTAMTTDD TEVPAMTLAP GHAALETQTL SAETSSRAST PAGPIPEAET RGAKRISPAR ETRSFTKTSP NFMVLIATSV ETSAASGSPE GAGMTTVQTI TGSDPREAIF DTLCTDDSSE EAKTLTMDIL TLAHTSTEAK GLSSESSASS DSPHPVITPS RASESSASSD GPHPVITPSR ASESSASSDG PHPVITPSRA SESSASSDGP HPVITPSRAS ESSASSDGPH PVITPSRASE SSASSDGPHP VITPSRASES SASSDGPHPV ITPSRASESS ASSDGPHPVI TPSRASESSA SSDGPHPVIT PSRASESSAS SDGLHPVITP SRASESSASS DGPHPVITPS RASESSASSD GPHPVITPSW SPGSDVTLLA EALVTVTNIE VINCSITEIE TTTSSIPGAS DTDLIPTEGV KASSTSDPPA LPDSTEAKPH ITEVTASAET LSTAGTTESA APDATVGTPL PTNSATEREV TAPGATTLSG ALVTVSRNPL EETSALSVET PSYVKVSGAA PVSIEAGSAV GKTTSFAGSS ASSYSPSEAA LKNFTPSETP TMDIATKGPF PTSRDPLPSV PPTTTNSSRG TNSTLAKITT SAKTTMKPPT ATPTTARTRP TTDVSAGENG GFLLLRLSVA SPEDLTDPRV AERLMQQLHR ELHAHAPHFQ VSLLRVRRG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MUC20重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际引用请核实)：
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1. **文献名称**：*"Recombinant MUC20 suppresses EGFR signaling in renal carcinoma cells"*
**作者**：Yamada T, et al.
**摘要**：研究通过重组MUC20蛋白在肾癌细胞中的表达，发现其通过抑制EGFR-MAPK信号通路抑制肿瘤增殖，提示MUC20可能作为肿瘤抑制因子发挥作用。
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2. **文献名称**：*"Expression and purification of human MUC20 recombinant protein in mammalian cells for functional studies"*
**作者**：Chen L, et al.
**摘要**：描述了利用哺乳动物表达系统高效表达并纯化功能性MUC20重组蛋白的方法，验证了其与肝细胞生长因子受体(Met)的相互作用，为后续机制研究提供工具。
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3. **文献名称**：*"MUC20 recombinant protein modulates macrophage polarization in chronic inflammation"*
**作者**：Kim S, et al.
**摘要**：发现重组MUC20蛋白可通过调节TLR4/NF-κB通路促进巨噬细胞向抗炎表型转化，为炎症性疾病治疗提供潜在靶点。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词“MUC20 recombinant protein”或“MUC20 expression”。
**Background of MUC20 Recombinant Protein**
MUC20. a member of the mucin family, is a transmembrane glycoprotein encoded by the *MUC20* gene. Mucins are characterized by their heavily glycosylated structures and roles in cell adhesion, lubrication, and signaling. MUC20 is expressed in various tissues, including the kidneys, placenta, and immune cells, and is implicated in modulating cellular signaling pathways, particularly through interactions with receptor tyrosine kinases like MET (hepatocyte growth factor receptor).
Structurally, MUC20 contains a conserved N-terminal domain, a central mucin-like region with O-glycosylation sites, and a short cytoplasmic tail. Its extracellular domain facilitates ligand-receptor interactions, while the cytoplasmic domain influences intracellular signaling cascades. Dysregulation of MUC20 has been linked to pathological conditions, such as chronic kidney disease, cancer progression, and immune dysfunction. For instance, in renal fibrosis, MUC20 overexpression inhibits MET signaling, disrupting tissue repair mechanisms.
Recombinant MUC20 protein is engineered using expression systems (e.g., mammalian cells, *E. coli*) to produce soluble, bioactive forms lacking the transmembrane domain. This enables studies on its molecular interactions, receptor binding, and functional roles in vitro or in vivo. Researchers utilize MUC20 recombinant protein to explore its dual role as a tumor suppressor or promoter, depending on context, and its potential as a diagnostic biomarker or therapeutic target.
Current research focuses on clarifying its signaling mechanisms, glycosylation patterns, and therapeutic applications, particularly in diseases driven by aberrant growth factor signaling. Its recombinant form serves as a critical tool for advancing understanding of mucin biology and developing targeted therapies.
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