| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA5B |
| Uniprot No | Q9P283 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1151aa |
| 氨基酸序列 | MPCGFSPSPVAHHLVPGPPDTPAQQLRCGWTVGGWLLSLVRGLLPCLPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIHLVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS |
| 预测分子量 | 125,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMA5B重组蛋白的3篇参考文献的简要信息：
1. **文献名称**: "Semaphorin 5B mediates synapse elimination in hippocampal neurons"
**作者**: K. Oshima et al.
**摘要**: 该研究通过重组SEMA5B蛋白发现其通过结合神经连接蛋白(neurexin)调控海马神经元突触的修剪，揭示了其在神经回路发育中的关键作用。
2. **文献名称**: "SEMA5B promotes tumor angiogenesis in non-small cell lung cancer by regulating endothelial cell proliferation"
**作者**: Y. Chen et al.
**摘要**: 利用重组SEMA5B蛋白进行体外实验，发现其通过激活VEGFR2信号通路促进内皮细胞增殖，进而驱动非小细胞肺癌的血管生成。
3. **文献名称**: "Structural basis of SEMA5B recognition by plexin-B2 receptor"
**作者**: M. Zhang et al.
**摘要**: 通过重组SEMA5B胞外结构域与Plexin-B2受体的共结晶分析，阐明了二者结合的分子机制，为靶向该互作的药物设计提供了结构基础。
注：上述文献为示例性内容，实际研究中建议通过PubMed或Web of Science以"SEMA5B recombinant"为关键词检索最新论文。部分研究可能聚焦于SEMA5B的病理功能而非重组蛋白技术细节。
**Background of SEMA5B Recombinant Protein**
SEMA5B, a member of the semaphorin protein family, is a transmembrane glycoprotein involved in axonal guidance, cell migration, and tissue morphogenesis. Semaphorins are characterized by a conserved ~500-amino acid extracellular "sema domain" critical for receptor binding and signaling. SEMA5B uniquely combines this sema domain with thrombospondin repeats, enabling interactions with extracellular matrix components and cell surface receptors like plexins and integrins. These interactions modulate cytoskeletal dynamics and intracellular signaling pathways, influencing processes such as angiogenesis, immune regulation, and tumor progression.
Recombinant SEMA5B protein is engineered to study its structure-function relationships and therapeutic potential. Produced via mammalian or insect cell systems, it retains post-translational modifications (e.g., glycosylation) essential for biological activity. The protein is often expressed as a soluble fragment (e.g., extracellular domain) with affinity tags (His, Fc) to facilitate purification and detection.
Functionally, SEMA5B exhibits dual roles depending on cellular context. In cancers, it may act as a tumor suppressor by inhibiting metastasis or as an oncogene by promoting invasion via matrix metalloproteinase activation. In neural systems, SEMA5B regulates synapse formation and neuronal connectivity, with implications in neurodevelopmental disorders. Dysregulation of SEMA5B is linked to glioblastoma, colorectal cancer, and autism spectrum disorders.
Current research leverages recombinant SEMA5B to dissect its signaling mechanisms, screen for receptor antagonists/agonists, and explore its utility in regenerative medicine or targeted therapies. Challenges include resolving its paradoxical roles and optimizing delivery strategies for clinical applications.
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