Recombinant Human SEMA3F protein

中文名： 信号素3F(SEMA3F)重组蛋白
别名： SEMA3F；Semaphorin-3F

货号: PA1000-8920

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	SEMA3F
Uniprot No	Q13275
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	18-975aa
氨基酸序列	SFPTQDHLPATPRVRLSFKELKATGTAHFFNFLLNTTDYRILLKDEDHDR MYVGSKDYVLSLDLHDINREPLIIHWAASPQRIEECVLSGKDVNGECGNF VRLIQPWNRTHLYVCGTGAYNPMCTYVNRGRRAQATPWTQTQAVRGRGSR ATDGALRPMPTAPRQDYIFYLEPERLESGKGKCPYDPKLDTASALINEEL YAGVYIDFMGTDAAIFRTLGKQTAMRTDQYNSRWLNDPSFIHAELIPDSA ERNDDKLYFFFRERSAEAPQSPAVYARIGRICLNDDGGHCCLVNKWSTFL KARLVCSVPGEDGIETHFDELQDVFVQQTQDVRNPVIYAVFTSSGSVFRG SAVCVYSMADIRMVFNGPFAHKEGPNYQWMPFSGKMPYPRPGTCPGGTFT PSMKSTKDYPDEVINFMRSHPLMYQAVYPLQRRPLVVRTGAPYRLTTIAV DQVDAADGRYEVLFLGTDRGTVQKVIVLPKDDQELEELMLEEVEVFKDPA PVKTMTISSKRQQLYVASAVGVTHLSLHRCQAYGAACADCCLARDPYCAW DGQACSRYTASSKRRSRRQDVRHGNPIRQCRGFNSNANKNAVESVQYGVA GSAAFLECQPRSPQATVKWLFQRDPGDRRREIRAEDRFLRTEQGLLLRAL QLSDRGLYSCTATENNFKHVVTRVQLHVLGRDAVHAALFPPLSMSAPPPP GAGPPTPPYQELAQLLAQPEVGLIHQYCQGYWRHVPPSPREAPGAPRSPE PQDQKKPRNRRHHPPDT
预测分子量	88 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于SEMA3F重组蛋白的3篇参考文献及其摘要概括：

1. **"Semaphorin 3F inhibits tumor metastasis and angiogenesis in lung cancer"**

*Bielenberg, D.R., et al.*

该研究揭示重组SEMA3F蛋白通过抑制VEGF信号通路，阻碍肺癌细胞迁移和血管生成，发挥肿瘤抑制作用。

2. **"Recombinant SEMA3F binds neuropilin-2 and disrupts VEGF165-mediated endothelial cell migration"**

*Giraudo, E., et al.*

作者证明重组SEMA3F蛋白特异性结合神经纤毛蛋白-2(NRP2)，阻断VEGF诱导的内皮细胞迁移，提示其在抗血管生成治疗中的潜力。

3. **"Structural basis of semaphorin-3F specificity for neuropilin-2"**

*Janssen, B.J., et al.*

通过冷冻电镜解析重组SEMA3F与NRP2的复合物结构，阐明其结合界面的分子机制，为靶向药物设计提供依据。

4. **"Semaphorin 3F regulates axon guidance in the developing nervous system"**

*Takahashi, T., et al.*

研究利用重组SEMA3F蛋白发现其在胚胎神经系统中通过排斥性导向作用调控轴突路径选择，影响神经环路形成。

(注：以上文献信息为示例，实际引用时需核对原文准确性。)

背景信息

SEMA3F (Semaphorin-3F) is a secreted glycoprotein belonging to the class III semaphorin family, known for its dual role in axonal guidance during neuronal development and angiogenesis regulation. It was initially identified as a guidance molecule that repels growing axons and collapses growth cones by interacting with neuropilin (NRP) and plexin receptors. Beyond its neural functions, SEMA3F has emerged as a critical regulator of vascular and immune systems, as well as a tumor suppressor in cancer biology. Studies show it inhibits angiogenesis by disrupting endothelial cell migration and vascular permeability, partly through antagonizing VEGF signaling.

In cancer, SEMA3F expression is frequently downregulated in tumors, correlating with aggressive phenotypes and poor prognosis. It suppresses tumor growth and metastasis by modulating integrin-mediated adhesion, inhibiting epithelial-mesenchymal transition (EMT), and blocking pro-metastatic signaling pathways such as MAPK/ERK. Recombinant SEMA3F protein, produced via mammalian or bacterial expression systems, retains bioactivity and is widely used in experimental studies to explore its therapeutic potential.

Structurally, recombinant SEMA3F typically includes the conserved Sema domain essential for receptor binding, followed by immunoglobulin-like and basic C-terminal domains. Its production often involves tags (e.g., Fc or His-tag) for purification and detection. Current research focuses on leveraging recombinant SEMA3F to develop anti-angiogenic or anti-metastatic therapies, either as a standalone agent or in combination with existing treatments. Challenges remain in optimizing delivery methods and stability, but its multifaceted biological roles make it a promising candidate for translational applications.