| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THBS4 |
| Uniprot No | P35443 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-961aa |
| 氨基酸序列 | MLAPRGAAVLLLHLVLQRWLAAGAQATPQVFDLLPSSSQRLNPGALLPVLTDPALNDLYVISTFKLQTKSSATIFGLYSSTDNSKYFEFTVMGRLNKAILRYLKNDGKVHLVVFNNLQLADGRRHRILLRLSNLQRGAGSLELYLDCIQVDSVHNLPRAFAGPSQKPETIELRTFQRKPQDFLEELKLVVRGSLFQVASLQDCFLQQSEPLAATGTGDFNRQFLGQMTQLNQLLGEVKDLLRQQVKETSFLRNTIAECQACGPLKFQSPTPSTVVPPAPPAPPTRPPRRCDSNPCFRGVQCTDSRDGFQCGPCPEGYTGNGITCIDVDECKYHPCYPGVHCINLSPGFRCDACPVGFTGPMVQGVGISFAKSNKQVCTDIDECRNGACVPNSICVNTLGSYRCGPCKPGYTGDQIRGCKAERNCRNPELNPCSVNAQCIEERQGDVTCVCGVGWAGDGYICGKDVDIDSYPDEELPCSARNCKKDNCKYVPNSGQEDADRDGIGDACDEDADGDGILNEQDNCVLIHNVDQRNSDKDIFGDACDNCLSVLNNDQKDTDGDGRGDACDDDMDGDGIKNILDNCPKFPNRDQRDKDGDGVGDACDSCPDVSNPNQSDVDNDLVGDSCDTNQDSDGDGHQDSTDNCPTVINSAQLDTDKDGIGDECDDDDDNDGIPDLVPPGPDNCRLVPNPAQEDSNSDGVGDICESDFDQDQVIDRIDVCPENAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTQTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVKSKTGPGEHLRNSLWHTGDTSDQVRLLWKDSRNVGWKDKVSYRWFLQHRPQVGYIRVRFYEGSELVADSGVTIDTTMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQEFQTQNFDRFDN |
| 预测分子量 | 105,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于THBS4重组蛋白的3篇代表性文献，按研究领域分类整理：
1. **标题**：Recombinant THBS4 promotes hepatocellular carcinoma metastasis via TGF-β/Smad2 signaling
**作者**：Li, X., Zhang, Y., & Wang, H.
**摘要**：该研究通过大肠杆菌系统表达重组THBS4蛋白，发现其激活TGF-β/Smad2通路，促进肝癌细胞EMT转化和体内转移，提示THBS4可作为肝癌治疗靶点。
2. **标题**：THBS4 recombinant protein attenuates myocardial fibrosis through modulation of macrophage polarization
**作者**：Chen, J., Liu, R., & Zhou, M.
**摘要**：利用CHO细胞表达的重组THBS4可调控巨噬细胞向M2型极化，抑制心肌成纤维细胞活化，改善小鼠心肌纤维化模型的心脏功能，为心血管疾病提供新治疗策略。
3. **标题**：Engineering a THBS4-based bioactive coating for bone regeneration implants
**作者**：Kim, S., Park, J.H., & Lee, W.
**摘要**：开发了搭载重组THBS4的钛合金植入体涂层，实验显示其通过整合素α3β1受体增强成骨细胞粘附和分化，显著提升大鼠颅骨缺损模型的骨再生效率。
注：以上文献信息为模拟生成，实际引用需查询具体论文数据库。建议通过PubMed/Google Scholar搜索关键词“recombinant THBS4”或结合研究领域筛选(如“THBS4 cancer”或“THBS4 tissue engineering”)，重点关注近5年发表的机制研究或应用探索类论文。
**Background of THBS4 Recombinant Protein**
Thrombospondin-4 (THBS4), a member of the thrombospondin protein family, is a multifunctional extracellular matrix glycoprotein involved in cell adhesion, migration, and tissue remodeling. The THBS family comprises five members (THBS1-5), with THBS4 sharing structural motifs such as N-terminal domains, properdin-like repeats, and calcium-binding regions. Unlike other thrombospondins, THBS4 is predominantly expressed in cardiac and skeletal muscle, though it is also detectable in other tissues during development or injury. Its expression is regulated by mechanical stress, hypoxia, and inflammatory cytokines, linking it to adaptive responses in tissue repair and disease.
Recombinant THBS4 is produced via genetic engineering, often using mammalian or insect cell systems to ensure proper post-translational modifications. This engineered protein retains functional domains critical for interactions with integrins, growth factors (e.g., TGF-β), and extracellular matrix components like collagen. Researchers utilize recombinant THBS4 to study its roles in modulating cellular signaling pathways, such as ERK and Akt, which influence cell survival, fibrosis, and inflammation.
In disease contexts, THBS4 is implicated in cardiac hypertrophy, muscular dystrophy, and cancer. For example, elevated THBS4 levels correlate with myocardial remodeling in cardiomyopathy, suggesting its role as a biomarker or therapeutic target. Paradoxically, THBS4 exhibits dual roles in cancer—promoting tumor progression in some contexts (e.g., colorectal cancer) while suppressing metastasis in others (e.g., pancreatic cancer), likely dependent on microenvironmental factors.
Current research focuses on deciphering THBS4’s context-dependent mechanisms and therapeutic potential. Its recombinant form enables mechanistic studies, drug screening, and exploration of regenerative medicine applications. However, unresolved questions remain about its precise signaling partners and disease-specific functions, highlighting the need for further investigation.
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