Recombinant Human TAS1R2 protein

中文名： 味觉受体1型成员2(TAS1R2)重组蛋白
别名： TAS1R2；GPR71；T1R2；TR2；Taste receptor type 1 member 2

货号: PA1000-8980

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	TAS1R2
Uniprot No	Q8TE23
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	20-566aa
氨基酸序列	EPAENSDFYLPGDYLLGGLFSLHANMKGIVHLNFLQVPMCKEYEVKVIGYNLMQAMRFAVEEINNDSSLLPGVLLGYEIVDVCYISNNVQPVLYFLAHEDNLLPIQEDYSNYISRVVAVIGPDNSESVMTVANFLSLFLLPQITYSAISDELRDKVRFPALLRTTPSADHHIEAMVQLMLHFRWNWIIVLVSSDTYGRDNGQLLGERVARRDICIAFQETLPTLQPNQNMTSEERQRLVTIVDKLQQSTARVVVVFSPDLTLYHFFNEVLRQNFTGAVWIASESWAIDPVLHNLTELRHLGTFLGITIQSVPIPGFSEFREWGPQAGPPPLSRTSQSYTCNQECDNCLNATLSFNTILRLSGERVVYSVYSAVYAVAHALHSLLGCDKSTCTKRVVYPWQLLEEIWKVNFTLLDHQIFFDPQGDVALHLEIVQWQWDRSQNPFQSVASYYPLQRQLKNIQDISWHTINNTIPMSMCSKRCQSGQKKKPVGIHVCCFECIDCLPGTFLNHTEDEYECQACPNNEWSYQSETSCFKRQLVFLEWHEAPT
预测分子量	67.6 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于 TAS1R2 重组蛋白的 3 篇代表性文献，涵盖其结构、功能和应用研究：

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1. **文献名称**: *"Sweet taste receptor TAS1R2/TAS1R3 heterodimer: Structural insights into ligand binding and signaling mechanisms"*

**作者**: Jiang, P., Cui, M., & Zhao, B.

**摘要**: 该研究利用冷冻电镜技术解析了人类甜味受体 TAS1R2/TAS1R3 异源二聚体的三维结构，揭示了天然甜味剂(如蔗糖)和人造甜味剂(如阿斯巴甜)的结合位点差异，并阐明了其激活下游信号通路的分子机制。

2. **文献名称**: *"Functional expression of TAS1R2 sweet receptor in HEK293 cells for high-throughput screening of sweeteners"*

**作者**: Nelson, G., Hoon, M.A., & Chandrashekar, J.

**摘要**: 研究团队在 HEK293 细胞中成功重组表达了 TAS1R2/TAS1R3 受体，结合钙离子荧光成像技术建立高通量筛选平台，用于快速鉴定新型甜味化合物，并验证了受体对不同甜味分子的特异性响应。

3. **文献名称**: *"Species-specific differences in sweet taste receptor TAS1R2 responsiveness to ligands"*

**作者**: Masuda, K., Koizumi, A., & Nakamura, Y.

**摘要**: 通过比较人类、小鼠和食肉动物的 TAS1R2 重组蛋白功能，发现其甜味感知能力存在显著物种差异。例如，猫科动物的 TAS1R2 因关键结构域缺失无法结合甜味分子，解释了其食肉特性的演化机制。

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**备注**：以上文献为示例性内容，实际引用时需根据具体研究领域补充真实发表的论文(可通过 PubMed 或 Web of Science 检索关键词 "TAS1R2 recombinant protein" 或 "TAS1R2/TAS1R3 heterodimer" 获取)。

背景信息

**Background of TAS1R2 Recombinant Protein**

TAS1R2. a member of the taste receptor type 1 (T1R) family, is a Class C G protein-coupled receptor (GPCR) critical for sweet taste perception. It functions as a heterodimer with TAS1R3. forming a sweet taste receptor complex that detects natural sugars, artificial sweeteners, and some amino acids. This receptor is expressed primarily in taste buds on the tongue, where it initiates intracellular signaling cascades upon ligand binding, ultimately transmitting sweet taste signals to the brain.

The recombinant TAS1R2 protein is engineered through molecular cloning and expressed in heterologous systems (e.g., HEK293 cells) to study its structure, ligand interactions, and signaling mechanisms. Recombinant production enables precise control over protein purity and functionality, bypassing challenges associated with isolating native receptors from tissues.

Research on TAS1R2 has broad implications. It aids in understanding taste physiology, dietary preferences, and metabolic disorders linked to sugar consumption. Additionally, it supports drug discovery for obesity and diabetes by identifying sweet taste modulators. In the food industry, recombinant TAS1R2 is used to screen novel sweeteners or taste enhancers with reduced caloric impact.

Structural studies of TAS1R2. often combined with computational modeling, reveal ligand-binding domains and activation mechanisms. However, challenges persist, such as maintaining receptor stability during purification and reproducing native post-translational modifications. Advances in cell-free expression systems and glycosylation engineering aim to address these limitations.

Overall, TAS1R2 recombinant protein serves as a vital tool for dissecting sweet taste biology and developing applications in nutrition, medicine, and biotechnology.