| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARX |
| Uniprot No | Q96QS3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-562aa |
| 氨基酸序列 | MSNQYQEEGC SERPECKSKS PTLLSSYCID SILGRRSPCK MRLLGAAQSL PAPLTSRADP EKAVQGSPKS SSAPFEAELH LPPKLRRLYG PGGGRLLQGA AAAAAAAAAA AAAAATATAG PRGEAPPPPP PTARPGERPD GAGAAAAAAA AAAAAWDTLK ISQAPQVSIS RSKSYRENGA PFVPPPPALD ELGGPGGVTH PEERLGVAGG PGSAPAAGGG TGTEDDEEEL LEDEEDEDEE EELLEDDEEE LLEDDARALL KEPRRCPVAA TGAVAAAAAA AVATEGGELS PKEELLLHPE DAEGKDGEDS VCLSAGSDSE EGLLKRKQRR YRTTFTSYQL EELERAFQKT HYPDVFTREE LAMRLDLTEA RVQVWFQNRR AKWRKREKAG AQTHPPGLPF PGPLSATHPL SPYLDASPFP PHHPALDSAW TAAAAAAAAA FPSLPPPPGS ASLPPSGAPL GLSTFLGAAV FRHPAFISPA FGRLFSTMAP LTSASTAAAL LRQPTPAVEG AVASGALADP ATAAADRRAS SIAALRLKAK EHAAQLTQLN ILPGTSTGKE VC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARX重组蛋白的3篇参考文献及其摘要概述：
1. **文献名称**：*Functional analysis of ARX mutations in protein stability and transcriptional activity*
**作者**：Collombat P, et al.
**摘要**：该研究通过构建ARX重组蛋白突变体，分析其在胚胎干细胞中的稳定性及对下游靶基因的调控作用，揭示了ARX蛋白关键结构域(如C端多丙氨酸重复序列)突变如何导致转录活性丧失，并与X连锁智力障碍发病相关。
2. **文献名称**：*Recombinant ARX homeobox protein regulates pancreatic α-cell differentiation*
**作者**：Gage BK, et al.
**摘要**：研究利用重组ARX蛋白在小鼠胰腺祖细胞模型中进行功能实验，证明ARX通过抑制胰岛素表达并激活胰高血糖素通路，主导胰腺α细胞分化，为糖尿病细胞治疗提供分子机制依据。
3. **文献名称**：*Structural insights into ARX-DNA interactions by recombinant protein crystallography*
**作者**：Shoubridge C, et al.
**摘要**：通过表达纯化人源ARX重组蛋白并进行X射线晶体学分析，解析了ARX同源结构域与靶DNA结合的分子模式，发现特定氨基酸残基对DNA结合特异性起关键作用，解释了部分致病突变的分子基础。
注：上述文献为示例，实际引用时需核实具体来源及发表年份。建议通过PubMed或Web of Science以“ARX recombinant protein”为关键词检索最新研究。
**Background of ARX Recombinant Protein**
The Aristaless-related homeobox (ARX) gene encodes a transcription factor critical for embryonic development, particularly in the brain, pancreas, and reproductive organs. As a member of the homeobox gene family, ARX regulates gene expression by binding to DNA, playing pivotal roles in cell differentiation, neuronal migration, and organogenesis. Mutations in ARX are linked to severe neurodevelopmental disorders, including X-linked lissencephaly, intellectual disabilities, epilepsy, and syndromes affecting pancreatic development, such as congenital hyperinsulinism.
Recombinant ARX protein is produced using genetic engineering techniques, where the ARX gene is cloned into expression vectors and expressed in host systems like *E. coli* or mammalian cells. This allows large-scale production of purified, functional ARX protein for research and therapeutic exploration. Its applications span studying molecular mechanisms of ARX-associated diseases, screening drugs to modulate ARX activity, and developing gene therapies. For instance, recombinant ARX enables *in vitro* studies of its interactions with co-factors or DNA targets, as well as *in vivo* models to assess its role in tissue development or disease rescue.
The development of ARX recombinant protein also addresses challenges in analyzing endogenous ARX, which is low in abundance and difficult to isolate natively. By providing a consistent and scalable source, recombinant ARX accelerates insights into its structure-function relationships and dysregulation in pathologies. Furthermore, it supports regenerative medicine efforts, such as guiding stem cell differentiation into ARX-dependent lineages. Overall, ARX recombinant protein serves as a vital tool bridging genetic knowledge to translational advancements in neurodevelopmental and metabolic disorders.
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