| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEPE |
| Uniprot No | Q9NQ76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-525aa |
| 氨基酸序列 | PTF QPQTEKTKQS CVEEQRQEEK NKDNIGFHHL GKRINQELSS KENIVQERKK DLSLSEASEN KGSSKSQNYF TNRQRLNKEY SISNKENTHN GLRMSIYPKS TGNKGFEDGD DAISKLHDQE EYGAALIRNN MQHIMGPVTA IKLLGEENKE NTPRNVLNII PASMNYAKAH SKDKKKPQRD SQAQKSPVKS KSTHRIQHNI DYLKHLSKVK KIPSDFEGSG YTDLQERGDN DISPFSGDGQ PFKDIPGKGE ATGPDLEGKD IQTGFAGPSE AESTHLDTKK PGYNEIPERE ENGGNTIGTR DETAKEADAV DVSLVEGSND IMGSTNFKEL PGREGNRVDA GSQNAHQGKV EFHYPPAPSK EKRKEGSSDA AESTNYNEIP KNGKGSTRKG VDHSNRNQAT LNEKQRFPSK GKSQGLPIPS RGLDNEIKNE MDSFNGPSHE NIITHGRKYH YVPHRQNNST RNKGMPQGKG SWGRQPHSNR RFSSRRRDDS SESSDSGSSS ESDGD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MEPE重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Matrix extracellular phosphoglycoprotein (MEPE) regulates osteoblast differentiation through a direct interaction with bone morphogenetic protein-2 (BMP-2)"*
**作者**：Rowe PS et al.
**摘要**：研究揭示了重组MEPE蛋白通过直接结合BMP-2抑制成骨细胞分化，表明其在骨代谢中的调控作用，可能为骨质疏松治疗提供新靶点。
2. **文献名称**：*"Recombinant MEPE protein modulates phosphate homeostasis by regulating renal phosphate reabsorption"*
**作者**：Bowe AE et al.
**摘要**：通过动物实验证明，重组MEPE蛋白能抑制肾小管对磷酸盐的重吸收，调节血清磷酸盐水平，提示其在慢性肾病矿物质代谢紊乱中的潜在应用。
3. **文献名称**：*"Structural and functional characterization of recombinant MEPE: Insights into its role in hypophosphatemic disorders"*
**作者**：Gattineni J et al.
**摘要**：解析了重组MEPE蛋白的晶体结构，并发现其通过ASARM肽段调控矿化过程，为低磷血症相关骨骼疾病的分子机制提供了新见解。
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以上文献均聚焦于重组MEPE蛋白的功能研究，涵盖骨代谢调控、磷酸盐稳态及结构分析等领域。
Matrix Extracellular Phosphoglycoprotein (MEPE) is a member of the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family, which plays critical roles in bone and tooth mineralization, phosphate regulation, and cellular signaling. Initially identified in tumors associated with oncogenic osteomalacia, MEPE is highly expressed in osteocytes, odontoblasts, and certain cancer tissues. It contains functional domains such as an RGD motif for cell adhesion and an acidic serine- and aspartate-rich motif (ASARM) involved in inhibiting hydroxyapatite crystal growth, thus modulating mineral metabolism. MEPE also interacts with fibroblast growth factor 23 (FGF23) to influence renal phosphate excretion, linking it to disorders like hypophosphatemic rickets and chronic kidney disease.
Recombinant MEPE is produced using genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems to ensure proper post-translational modifications. The protein is purified via affinity chromatography, often tagged with markers like His or Fc for detection and solubility. Its recombinant form enables detailed study of bone remodeling mechanisms, pathological calcification, and crosstalk between bone and kidney in phosphate homeostasis. Researchers utilize it to develop therapeutic strategies for skeletal diseases, including osteoporosis and mineralization disorders, as well as to explore its dual role in cancer progression—either suppressing metastasis or promoting tumor growth depending on context. Additionally, MEPE’s ASARM peptide has potential as a biomarker or therapeutic target for metabolic bone diseases. Overall, recombinant MEPE serves as a vital tool for unraveling mineralized tissue biology and related pathologies.
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