| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZP2 |
| Uniprot No | Q05996 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-736aa |
| 氨基酸序列 | MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSGNSIDVSQLVNPAFP GTVTCDEREITVEFPSSPGTKKWHASVVDPLGLDMPNCTYILDPEKLTLR ATYDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPAMQVEETQGLS ASTICQKDFMSFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLP EAMKEGFSLLIDNHRMTFHVPFNATGVTHYVQGNSHLYMVSLKLTFISPG QKVIFSSQAICAPDPVTCNATHMTLTIPEFPGKLKSVSFENQNIDVSQLH DNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQFYLASLKLTFLLRPETV SMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALDLGTLRVGN SSCQPVFEAQSQGLVRFHIPLNGCGTRYKFEDDKVVYENEIHALWTDFPP SKISRDSEFRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPY GENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQW NVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSS LVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGP ILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTA GDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH |
| 预测分子量 | 82,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZP2重组蛋白的3篇代表性文献的简要列举(注：文献为示例性概括，非真实存在)：
1. **文献名称**：*"Expression and purification of recombinant human ZP2 protein for structural analysis"*
**作者**：Smith A, et al.
**摘要**：本研究利用哺乳动物细胞表达系统成功表达并纯化了重组人ZP2蛋白，通过X射线晶体学解析其三维结构，揭示了ZP2在透明带中的关键功能域及其与精子相互作用的潜在机制。
2. **文献名称**：*"Role of recombinant ZP2 in sperm-egg binding: Insights from in vitro fertilization assays"*
**作者**：Chen L, et al.
**摘要**：通过体外受精实验证明，重组ZP2蛋白能够特异性结合精子表面受体，阻断其与卵母细胞的黏附，提示ZP2在受精过程中起关键作用，为开发非激素类避孕疫苗提供了理论基础。
3. **文献名称**：*"Immunocontraceptive potential of recombinant ZP2 protein in a murine model"*
**作者**：Kumar R, et al.
**摘要**：在小鼠模型中评估重组ZP2蛋白的免疫避孕效果，发现其可诱导高滴度抗体，显著降低生育率且无卵巢病理损伤，为基于ZP2的避孕疫苗研发提供了实验依据。
4. **文献名称**：*"Glycosylation modification of recombinant ZP2 protein enhances its stability and bioactivity"*
**作者**：Tanaka Y, et al.
**摘要**：通过优化CHO细胞培养条件实现重组ZP2蛋白的糖基化修饰，显著提高其稳定性和精子结合活性，为大规模生产功能性ZP2蛋白用于生殖医学研究奠定了基础。
(注：以上文献信息为模拟内容，实际研究中请通过PubMed或Web of Science等数据库检索具体文献。)
**Background of ZP2 Recombinant Protein**
Zona pellucida protein 2 (ZP2) is a critical glycoprotein component of the zona pellucida (ZP), an extracellular matrix surrounding mammalian oocytes and preimplantation embryos. The ZP plays essential roles in oocyte development, fertilization, and early embryonic protection. Among the four primary ZP glycoproteins (ZP1–4 in humans; ZP1–3 in mice), ZP2 is particularly significant for its involvement in sperm binding and the structural integrity of the ZP. During fertilization, ZP2 interacts with sperm surface receptors, mediating species-specific recognition and secondary sperm binding post-acrosome reaction.
Biologically, ZP2 is synthesized as a precursor protein that undergoes proteolytic processing to form mature, functional subunits. Structurally, it contains a conserved zona pellucida domain (ZP domain), which facilitates polymerization and matrix assembly. Mutations or deficiencies in ZP2 are linked to female infertility in humans and animal models, underscoring its reproductive importance.
Recombinant ZP2 protein is produced using genetic engineering techniques, often expressed in mammalian cell systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications, such as glycosylation, which are critical for its biological activity. The recombinant form enables detailed studies of ZP2’s molecular functions, including its role in sperm-egg interactions, ZP matrix formation, and immune responses.
Research applications of ZP2 recombinant protein span reproductive biology, contraceptive vaccine development, and infertility diagnostics. It serves as a tool to investigate ZP2-specific antibody responses, evaluate immunocontraceptive strategies, and model pathogenic mutations. Additionally, recombinant ZP2 aids in developing assays for oocyte quality assessment and improving assisted reproductive technologies.
Overall, ZP2 recombinant protein provides a controlled, ethical alternative to native ZP2 isolation, advancing both basic science and clinical innovations in reproductive health.
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