Recombinant Human HIPK2 protein

中文名： 同源域相互作用蛋白激酶2(HIPK2)重组蛋白
别名： HIPK2；Homeodomain-interacting protein kinase 2

货号: PA1000-9130

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	HIPK2
Uniprot No	Q9H2X6
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-640aa
氨基酸序列	MAPVYEGMASHVQVFSPHTLQSSAFCSVKKLKIEPSSNWDMTGYGSHSKV YSQSKNIPLSQPATTTVSTSLPVPNPSLPYEQTIVFPGSTGHIVVTSASS TSVTGQVLGGPHNLMRRSTVSLLDTYQKCGLKRKSEEIENTSSVQIIEEH PPMIQNNASGATVATATTSTATSKNSGSNSEGDYQLVQHEVLCSMTNTYE VLEFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARL STESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKY IRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSA SHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPL YPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDTDSPYPLWRLKT PDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADRREF IDLLKKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEI CKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQAPSSTS ATISLANPEVSILNYPSTLYQPSAASMAAVAQRSMPLQTG
预测分子量	98kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于HIPK2重组蛋白的假设性参考文献示例(仅供格式参考，非真实文献)：

1. **文献名称**：*"Expression and Purification of Recombinant HIPK2 Kinase Domain in Escherichia coli for Functional Studies"*

**作者**：Zhang Y. et al.

**摘要**：研究报道了利用大肠杆菌表达系统高效表达并纯化HIPK2激酶结构域重组蛋白，通过优化表达条件获得可溶性蛋白，并验证其体外激酶活性及底物磷酸化能力。

2. **文献名称**：*"Structural Analysis of HIPK2 Reveals Key Residues for Substrate Recognition"*

**作者**：Rodriguez M. et al.

**摘要**：通过X射线晶体学解析了重组HIPK2激酶结构域的3D结构，揭示了其与底物结合的活性位点特征，为设计靶向HIPK2的抑制剂提供结构基础。

3. **文献名称**：*"HIPK2 Recombinant Protein Modulates p53-dependent Apoptosis in Cancer Cell Lines"*

**作者**：Lee S. et al.

**摘要**：利用哺乳动物细胞表达的重组HIPK2蛋白，证明其通过磷酸化p53增强肿瘤细胞凋亡，揭示了HIPK2在DNA损伤应答中的分子机制。

4. **文献名称**：*"Development of a High-throughput Screening Assay for HIPK2 Inhibitors Using Recombinant Protein"*

**作者**：Johnson K. et al.

**摘要**：构建基于重组HIPK2蛋白的体外激酶活性检测体系，用于高通量筛选小分子抑制剂，发现多个潜在抗肿瘤候选化合物。

**注意**：以上为示例，实际引用时请查询真实文献(可参考PubMed、ScienceDirect等平台，关键词：HIPK2 recombinant protein, kinase activity, purification)。

背景信息

HIPK2 (Homeodomain-interacting protein kinase 2) is a serine/threonine kinase belonging to the CMGC kinase family, initially identified for its interaction with homeodomain transcription factors. It plays multifaceted roles in cellular stress responses, DNA damage repair, apoptosis, and regulation of signaling pathways such as p53. Wnt/β-catenin, and TGF-β. HIPK2 is activated by various stressors, including oxidative stress and hypoxia, and acts as a tumor suppressor by modulating transcriptional activity through phosphorylation of targets like p53. enhancing their pro-apoptotic functions. Dysregulation of HIPK2 is implicated in cancer, fibrosis, and neurodegenerative diseases, highlighting its therapeutic potential.

Recombinant HIPK2 protein, produced via heterologous expression systems (e.g., bacteria, insect, or mammalian cells), enables mechanistic studies of its kinase activity, substrate interactions, and structural features. Purification often involves affinity tags (e.g., GST, His-tag) for streamlined isolation. Researchers use recombinant HIPK2 to explore its role in pathways like apoptosis (via caspase activation) or fibrosis (via SMAD signaling), screen kinase inhibitors, and analyze mutations affecting enzymatic activity or protein stability. Mutant variants (kinase-dead or disease-associated) help dissect functional domains, while tagged versions aid in protein interaction mapping. Its application extends to disease models, drug discovery, and understanding post-translational modifications (e.g., phosphorylation, ubiquitination) that regulate HIPK2 stability and localization. Overall, recombinant HIPK2 serves as a critical tool for unraveling its biological significance and therapeutic targeting in human diseases.