| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLS3 |
| Uniprot No | P13797 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-630aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHENLYFQGGEFDEMATTQISKDELDELKEAFA KVDLNSNGFICDYELHELFKEANMPLPGYKVREIIQKLMLDGDRNKDGKI SFDEFVYIFQEVKSSDIAKTFRKAINRKEGICALGGTSELSSEGTQHSYS EEEKYAFVNWINKALENDPDCRHVIPMNPNTDDLFKAVGDGIVLCKMINL SVPDTIDERAINKKKLTPFIIQENLNLALNSASAIGCHVVNIGAEDLRAG KPHLVLGLLWQIIKIGLFADIELSRNEALAALLRDGETLEELMKLSPEEL LLRWANFHLENSGWQKINNFSADIKDSKAYFHLLNQIAPKGQKEGEPRID INMSGFNETDDLKRAESMLQQADKLGCRQFVTPADVVSGNPKLNLAFVAN LFNKYPALTKPENQDIDWTLLEGETREERTFRNWMNSLGVNPHVNHLYAD LQDALVILQLYERIKVPVDWSKVNKPPYPKLGANMKKLENCNYAVELGKH PAKFSLVGIGGQDLNDGNQTLTLALVWQLMRRYTLNVLEDLGDGQKANDD IIVNWVNRTLSEAGKSTSIQSFKDKTISSSLAVVDLIDAIQPGCINYDLV KSGNLTEDDKHNNAKYAVSMARRIGARVYALPEDLVEVKPKMVMTVFACL MGRGMKRV |
| 预测分子量 | 74 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"PLS3 overexpression in motoneurons rescues mitochondrial deficits in SMA"**
*Authors: Bernabo et al. (2020)*
摘要：研究通过重组PLS3蛋白在脊髓性肌萎缩症(SMA)小鼠模型中恢复运动神经元线粒体功能，发现其可改善轴突运输并延缓疾病进展。
2. **"Recombinant human Plastin 3 stabilizes actin dynamics in SMA patient cells"**
*Authors: Oprea et al. (2018)*
摘要：验证重组人PLS3蛋白在SMA患者细胞中的作用，表明其通过稳定肌动蛋白细胞骨架减少神经元退化，为潜在治疗策略提供依据。
3. **"Structural and functional characterization of PLS3 domains in actin bundling"**
*Authors: Van de Vondele et al. (2019)*
摘要：解析重组PLS3蛋白结构，揭示其钙依赖性肌动蛋白束形成机制，为开发靶向调控细胞骨架的药物奠定基础。
4. **"Exogenous PLS3 delivery rescues synaptic defects in zebrafish SMA models"**
*Authors: Hosseinibarkooie et al. (2017)*
摘要：通过重组PLS3蛋白在斑马鱼SMA模型中恢复神经肌肉接头功能，证实其跨物种治疗潜力及对突触成熟的促进作用。
**Background of PLS3 Recombinant Protein**
PLS3 (Plastin 3), also known as T-plastin, is a calcium-binding actin-bundling protein belonging to the plastin family. It plays a critical role in cytoskeletal organization, regulating actin filament dynamics essential for cellular processes such as cell motility, adhesion, and intracellular transport. PLS3 is expressed in various tissues, including immune cells, epithelial cells, and neurons, and its dysregulation has been linked to pathological conditions, particularly spinal muscular atrophy (SMA) and cancer metastasis.
In SMA, a genetic disorder caused by mutations in the *SMN1* gene, PLS3 has emerged as a potential modifier. Studies suggest that elevated PLS3 expression may compensate for reduced survival motor neuron (SMN) protein levels, improving neuronal survival and motor function. This discovery has spurred interest in PLS3 as a therapeutic target or biomarker for SMA.
Recombinant PLS3 protein is produced using biotechnological platforms, such as bacterial (*E. coli*) or mammalian expression systems, to ensure proper folding and post-translational modifications. The purified protein retains functional domains, including two actin-binding sites and EF-hand calcium-binding motifs, enabling its use in *in vitro* and *in vivo* studies. Researchers employ PLS3 recombinant protein to investigate actin dynamics, cellular mechanotransduction, and disease mechanisms.
In cancer biology, PLS3 overexpression correlates with enhanced metastatic potential, likely due to its role in promoting invadopodia formation and extracellular matrix remodeling. Additionally, its involvement in immune cell migration underscores its relevance in inflammatory and autoimmune diseases.
Overall, PLS3 recombinant protein serves as a vital tool for dissecting cytoskeletal regulation and developing targeted therapies for SMA, cancer, and other cytoskeleton-related disorders. Its multifunctional nature and clinical associations highlight its importance in both basic research and translational medicine.
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