Recombinant Human AQP8 protein

中文名： 水通道蛋白8(AQP8)重组蛋白
别名： AQP8；Aquaporin-8

货号: PA1000-9244

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	AQP8
Uniprot No	O94778
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-261aa
氨基酸序列	MSGEIAMCEPEFGNDKAREPSVGGRWRVSWYERFVQPCLVELLGSALFIFIGCLSVIENGTDTGLLQPALAHGLALGLVIATLGNISGGHFNPAVSLAAMLIGGLNLVMLLPYWVSQLLGGMLGAALAKAVSPEERFWNASGAAFVTVQEQGQVAGALVAEIILTTLLALAVCMGAINEKTKGPLAPFSIGFAVTVDILAGGPVSGGCMNPARAFGPAVVANHWNFHWIYWLGPLLAGLLVGLLIRCFIGDGKTRLILKAR
预测分子量	27,3 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于AQP8重组蛋白的3篇参考文献示例(注：内容为模拟概括，实际文献需根据具体研究补充)：

1. **文献名称**：*"Functional characterization of recombinant human AQP8 water channel protein"*

**作者**：Ishibashi K, et al.

**摘要**：通过在大肠杆菌中表达并纯化人源AQP8重组蛋白，发现其具有选择性水通透性，且对汞抑制敏感，提示其在细胞渗透调节中的潜在作用。

2. **文献名称**：*"AQP8 facilitates hydrogen peroxide permeability and modulates cellular oxidative stress"*

**作者**：Verkman AS, et al.

**摘要**：研究利用哺乳动物细胞重组表达AQP8.发现其可转运过氧化氢(H₂O₂)，提示AQP8在调节细胞内氧化还原状态中的生理意义。

3. **文献名称**：*"Expression and localization of AQP8 in gastrointestinal tract: Role in water absorption"*

**作者**：Laforenza U, et al.

**摘要**：通过重组蛋白技术在小肠上皮细胞中表达AQP8.证实其参与肠道跨膜水运输，可能影响腹泻或便秘等病理过程。

(如需更多文献，建议通过PubMed或Web of Science以“AQP8 recombinant”为关键词检索近年研究。)

背景信息

Aquaporin-8 (AQP8) is a member of the aquaporin family, a class of integral membrane proteins that facilitate selective water transport across cellular membranes. First identified in the late 1990s, AQP8 is distinguished by its unique permeability profile, primarily allowing water and, to a lesser extent, other small neutral molecules like urea or ammonia. Structurally, it shares the conserved aquaporin fold—six transmembrane helices and two asparagine-proline-alanine (NPA) motifs forming the water channel. Unlike some aquaporins (e.g., AQP4 or AQP1), AQP8 lacks glycerol transport capability, reflecting functional specialization.

Tissue-specific expression of AQP8 is observed in organs with high water flux demands, including the liver, pancreas, kidneys, testes, and gastrointestinal tract. In hepatocytes, it regulates bile production and osmoregulation; in pancreatic acinar cells, it influences enzyme secretion; in the colon, it mediates water absorption. AQP8 also localizes to mitochondria in certain tissues, suggesting a role in cellular energy metabolism or oxidative stress response, though its exact mitochondrial function remains under investigation.

Pathologically, AQP8 dysregulation has been linked to diseases such as cholestasis, pancreatitis, and male infertility. Its involvement in fluid homeostasis makes it a potential biomarker or therapeutic target. Recombinant AQP8 protein, typically produced in Escherichia coli or mammalian expression systems, enables mechanistic studies. Researchers employ it to dissect transport kinetics, structural dynamics, and regulatory pathways (e.g., phosphorylation or pH-dependent gating). Additionally, recombinant AQP8 aids in antibody development for diagnostic assays and high-throughput drug screening to identify modulators for channel-related disorders. Despite progress, unanswered questions persist regarding its physiological regulators and disease-specific roles, driving ongoing research in membrane biology and translational medicine.