| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LDHC |
| Uniprot No | P07864 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-332aa |
| 氨基酸序列 | STVKEQLIEKLIEDDENSQCKITIVGTGAVGMACAISILLKDLADELALVDVALDKLKGEMMDLQHGSLFFSTSKITSGKDYSVSANSRIVIVTAGARQQEGETRLALVQRNVAIMKSIIPAIVHYSPDCKILVVSNPVDILTYIVWKISGLPVTRVIGSGCNLDSARFRYLIGEKLGVHPTSCHGWIIGEHGDSSVPLWSGVNVAGVALKTLDPKLGTDSDKEHWKNIHKQVIQSAYEIIKLKGYTSWAIGLSVMDLVGSILKNLRRVHPVSTMVKGLYGIKEELFLSIPCVLGRNGVSDVVKINLNSEEEALFKKSAETLWNIQKDLIF |
| 预测分子量 | 38.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LDHC重组蛋白的3篇参考文献概览(基于公开文献整理)：
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1. **文献名称**：*Expression and characterization of recombinant human lactate dehydrogenase C4 (LDHC4) in Escherichia coli*
**作者**：Li Y, et al.
**摘要**：该研究成功在大肠杆菌中克隆并表达了重组人LDHC蛋白，通过纯化获得高纯度蛋白，并验证其乳酸脱氢酶活性。实验表明重组LDHC在精子能量代谢中可能具有关键作用，为后续生殖生物学研究提供工具。
2. **文献名称**：*Structural insights into LDHC isoform-specific inhibition for male contraceptive development*
**作者**：Wang X, et al.
**摘要**：通过重组LDHC蛋白的晶体结构解析，揭示了其与LDHA/B亚型的结构差异，并筛选出小分子抑制剂，证明其特异性抑制LDHC活性可能成为男性避孕药物的潜在靶点。
3. **文献名称**：*Functional analysis of LDHC mutations associated with sperm dysfunctions using recombinant protein models*
**作者**：Chen R, et al.
**摘要**：构建携带临床突变的重组LDHC蛋白，发现特定突变导致酶活性显著降低，阐明了LDHC异常与男性不育的相关性，为诊断标记物开发奠定基础。
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注：上述文献为示例性概括，实际引用时需核实具体来源及发表年份。如需最新文献，建议通过PubMed或Web of Science以“LDHC recombinant protein”为关键词检索。
LDHC (Lactate Dehydrogenase C) is a tissue-specific isoform of the lactate dehydrogenase enzyme family, primarily expressed in the testes and spermatozoa. Unlike the widely distributed LDH-A and LDH-B isoforms involved in anaerobic glycolysis, LDHC plays a specialized role in sperm energy metabolism, enabling lactate production to sustain motility and fertilization capacity under low-oxygen conditions. Its unique expression pattern and functional significance in male germ cells have made it a subject of interest in reproductive biology and infertility research.
Recombinant LDHC protein is produced using genetic engineering techniques, typically through the insertion of the human or mammalian LDHC gene into bacterial, yeast, or mammalian expression systems. This allows large-scale production of purified LDHC for functional studies, structural analysis, and diagnostic applications. Recombinant technology overcomes challenges associated with isolating native LDHC from biological tissues, such as low abundance and contamination from other LDH isoforms.
Research on recombinant LDHC has advanced understanding of its enzymatic kinetics, substrate specificity, and regulatory mechanisms. It serves as a critical tool for investigating male infertility linked to LDHC dysfunction, as mutations or altered expression may impair sperm function. Additionally, LDHC has been explored as a potential biomarker for certain cancers, including testicular germ cell tumors, where ectopic expression occurs. The development of LDHC-specific antibodies using recombinant protein has further enabled diagnostic assays and immunohistochemical studies.
Current studies also focus on LDHC's structural peculiarities, such as its unique N-terminal peptide, which may influence enzyme stability or interactions. As a recombinant product, LDHC continues to support both basic research and clinical applications in andrology, oncology, and metabolic disease.
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