| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MATN2 |
| Uniprot No | O00339 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-956aa |
| 氨基酸序列 | MEKMLAGCFLLILGQIVLLPAEARERSRGRSISRGRHARTHPQTALLESSCENKRADLVFIIDSSRSVNTHDYAKVKEFIVDILQFLDIGPDVTRVGLLQYGSTVKNEFSLKTFKRKSEVERAVKRMRHLSTGTMTGLAIQYALNIAFSEAEGARPLRENVPRVIMIVTDGRPQDSVAEVAAKARDTGILIFAIGVGQVDFNTLKSIGSEPHEDHVFLVANFSQIETLTSVFQKKLCTAHMCSTLEHNCAHFCINIPGSYVCRCKQGYILNSDQTTCRIQDLCAMEDHNCEQLCVNVPGSFVCQCYSGYALAEDGKRCVAVDYCASENHGCEHECVNADGSYLCQCHEGFALNPDKKTCTKIDYCASSNHGCQHECVNTDDSYSCHCLKGFTLNPDKKTCRRINYCALNKPGCEHECVNMEESYYCRCHRGYTLDPNGKTCSRVDHCAQQDHGCEQLCLNTEDSFVCQCSEGFLINEDLKTCSRVDYCLLSDHGCEYSCVNMDRSFACQCPEGHVLRSDGKTCAKLDSCALGDHGCEHSCVSSEDSFVCQCFEGYILREDGKTCRRKDVCQAIDHGCEHICVNSDDSYTCECLEGFRLAEDGKRCRRKDVCKSTHHGCEHICVNNGNSYICKCSEGFVLAEDGRRCKKCTEGPIDLVFVIDGSKSLGEENFEVVKQFVTGIIDSLTISPKAARVGLLQYSTQVHTEFTLRNFNSAKDMKKAVAHMKYMGKGSMTGLALKHMFERSFTQGEGARPLSTRVPRAAIVFTDGRAQDDVSEWASKAKANGITMYAVGVGKAIEEELQEIASEPTNKHLFYAEDFSTMDEISEKLKKGICEALEDSDGRQDSPAGELPKTVQQPTESEPVTINIQDLLSCSNFAVQHRYLFEEDNLLRSTQKLSHSTKPSGSPLEEKHDQCKCENLIMFQNLANEEVRKLTQRLEEMTQRMEALENRLRYR |
| 预测分子量 | 106 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MATN2重组蛋白的3篇示例参考文献(注：文献为虚构示例，实际文献需通过学术数据库检索)：
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1. **文献名称**: "Recombinant MATN2 promotes chondrocyte adhesion and cartilage repair in osteoarthritis models"
**作者**: Zhang L, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功制备了重组MATN2蛋白，并发现其能显著增强体外软骨细胞的黏附能力。动物实验表明，局部注射MATN2重组蛋白可促进骨关节炎模型中的软骨组织修复，提示其作为治疗剂的潜力。
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2. **文献名称**: "Structural characterization of human MATN2 reveals its role in extracellular matrix assembly"
**作者**: Müller S, et al.
**摘要**: 通过重组表达并纯化人源MATN2蛋白，结合X射线晶体学解析其多结构域三维构象。功能实验证实MATN2通过结合胶原纤维和蛋白聚糖参与细胞外基质(ECM)组装，为理解其调控组织弹性的分子机制提供依据。
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3. **文献名称**: "MATN2 as a novel biomarker in colorectal cancer: Recombinant protein-based serum analysis"
**作者**: Tanaka K, et al.
**摘要**: 开发基于重组MATN2蛋白的ELISA检测方法，发现结直肠癌患者血清中MATN2水平显著升高，且与肿瘤转移呈正相关。研究提示MATN2可能作为癌症诊断及预后的生物标志物。
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建议通过PubMed、Google Scholar等平台以“MATN2 recombinant protein”、“matrilin-2 expression”等关键词检索真实文献。
**Background of MATN2 Recombinant Protein**
Matrilin-2 (MATN2) is a member of the matrilin family, a group of extracellular matrix (ECM) proteins characterized by von Willebrand factor A (VWA) domains, epidermal growth factor (EGF)-like repeats, and coiled-coil oligomerization domains. MATN2 is broadly expressed in developing and adult tissues, particularly in cartilage, nervous systems, and connective tissues. It plays a role in ECM assembly, cell-matrix interactions, and tissue development by mediating interactions between collagen fibrils and other ECM components.
Recombinant MATN2 protein is engineered using biotechnological platforms (e.g., mammalian, insect, or bacterial expression systems) to produce purified, biologically active forms of the protein. Its production often involves cloning the MATN2 gene into expression vectors, followed by purification via affinity chromatography. The recombinant protein retains functional domains critical for binding partners like collagens, proteoglycans, and integrins, enabling studies on ECM organization, cellular adhesion, and signaling pathways.
Research highlights MATN2's dual roles in tissue homeostasis and disease. It supports tissue repair and regeneration by stabilizing ECM networks but has also been implicated in pathological conditions, including fibrosis, osteoarthritis, and cancers. For instance, MATN2 overexpression correlates with tumor progression by modulating integrin-mediated signaling and promoting metastasis.
Recombinant MATN2 serves as a vital tool for in vitro and in vivo studies, aiding in elucidating ECM biology, screening therapeutic targets, or developing biomaterials for regenerative medicine. Its applications extend to disease modeling, drug discovery, and understanding molecular mechanisms underlying ECM-related disorders. Ongoing studies aim to clarify its context-dependent functions and therapeutic potential in precision medicine.
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