| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LGI3 |
| Uniprot No | Q8N145 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-548aa |
| 氨基酸序列 | KRPPKTPPCP PSCSCTRDTA FCVDSKAVPR NLPSEVISLT LVNAAFSEIQ DGAFSHLPLL QFLLLNSNKF TLIGDNAFTG LSHLQYLFIE NNDIWALSKF TFRGLKSLTH LSLANNNLQT LPRDIFRPLD ILNDLDLRGN SLNCDCKVKW LVEWLAHTNT TVAPIYCASP PRFQEHKVQD LPLREFDCIT TDFVLYQTLA FPAVSAEPFL YSSDLYLALA QPGVSACTIL KWDYVERQLR DYDRIPAPSA VHCKPMVVDS QLYVVVAQLF GGSYIYHWDP NTTRFTRLQD IDPQRVRKPN DLEAFRIDGD WYFAVADSSK AGATSLYRWH QNGFYSHQAL HPWHRDTDLE FVDGEGKPRL IVSSSSQAPV IYQWSRTQKQ FVAQGEVTQV PDAQAVKHFR AGRDSYLCLS RYIGDSKILR WEGTRFSEVQ ALPSRGSLAL QPFLVGGRRY LALGSDFSFT QIYQWDEGRQ KFVRFQELAV QAPRAFCYMP AGDAQLLLAP SFKGQTLVYR HIVVDLSA |
| 预测分子量 | 61,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于LGI3重组蛋白的参考文献概要(基于公开研究数据模拟，非真实文献)：
1. **《LGI3 promotes neurite outgrowth by binding to ADAM22 and regulating synaptic protein expression》**
- **作者**: Suzuki K, et al.
- **摘要**: 研究通过重组LGI3蛋白在哺乳动物细胞中的表达，发现其通过与ADAM22受体结合激活下游信号通路，促进神经元突触蛋白(如NMDA受体)的表达，并增强神经突生长。
2. **《Recombinant LGI3 protein modulates cell adhesion and proliferation in keratinocytes via EGFR signaling》**
- **作者**: Wang Y, et al.
- **摘要**: 利用大肠杆菌表达系统纯化重组LGI3蛋白，发现其通过表皮生长因子受体(EGFR)信号通路调节角质形成细胞的黏附与增殖，提示LGI3在皮肤屏障修复中的潜在作用。
3. **《Structural and functional characterization of LGI3-EPTP complex in myelination》**
- **作者**: Kim HJ, et al.
- **摘要**: 通过体外重组LGI3与EPTP蛋白的相互作用实验，揭示LGI3通过形成复合物调控髓鞘形成相关基因的表达，为中枢神经系统发育研究提供新靶点。
如需具体文献，建议在PubMed或Google Scholar中以关键词“LGI3 recombinant protein”检索近期论文。
LGI3 (leucine-rich glioma-inactivated 3) is a secreted protein belonging to the LGI family, which includes four members (LGI1-4) characterized by leucine-rich repeats (LRR) and an EPTP (epitempin) domain. It is predominantly expressed in the central nervous system, particularly in neurons, and has been implicated in synaptic development, neuronal excitability, and cell adhesion. While its paralog LGI1 is well-studied for its role in autosomal dominant lateral temporal lobe epilepsy, LGI3 remains less understood, though emerging evidence suggests its involvement in neurodevelopmental and neurodegenerative processes.
Recombinant LGI3 protein is engineered using expression systems (e.g., mammalian cells, bacteria) to produce purified, bioactive forms for functional studies. Its structure includes conserved LRR domains, which mediate protein-protein interactions, and a C-terminal EPTP domain that may regulate secretion or receptor binding. Research indicates LGI3 interacts with synaptic adhesion molecules like ADAM22/23. potentially modulating synaptic transmission or axonal guidance. Dysregulation of LGI3 has been tentatively linked to neurological disorders, including epilepsy, autism spectrum disorders, and glioblastoma, though mechanistic insights are limited.
Current applications of recombinant LGI3 focus on elucidating its physiological roles through in vitro binding assays, neuronal culture models, and animal studies. It also serves as an antigen for antibody development to probe its expression patterns in tissues or cerebrospinal fluid. Challenges include clarifying its receptor partners, signaling pathways, and disease relevance. Advances in recombinant protein technology may accelerate its characterization, offering potential for diagnostic or therapeutic innovations in neurology.
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