| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPHLN1 |
| Uniprot No | Q8NEY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-458aa |
| 氨基酸序列 | MWSEGRYEYE RIPRERAPPR SHPSDGYNRL VNIVPKKPPL LDRPGEGSYN RYYSHVDYRD YDEGRSFSHD RRSGPPHRGD ESGYRWTRDD HSASRQPEYR DMRDGFRRKS FYSSHYARER SPYKRDNTFF RESPVGRKDS PHSRSGSSVS SRSYSPERSK SYSFHQSQHR KSVRPGASYK RQNEGNPERD KERPVQSLKT SRDTSPSSGS AVSSSKVLDK PSRLTEKELA EAASKWAAEK LEKSDESNLP EISEYEAGST APLFTDQPEE PESNTTHGIE LFEDSQLTTR SKAIASKTKE IEQVYRQDCE TFGMVVKMLI EKDPSLEKSI QFALRQNLHE IESAGQTWQQ VPPVRNTEMD HDGTPENEGE ETAQSAPQPP QAPQPLQPRK KRVRRTTQLR RTTGAPDITW GMLKKTTQEA ERILLRTQTP FTPENLFLAM LSVVHCNSRK DVKPENKQ |
| 预测分子量 | 52,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PPHLN1(Periphilin 1)重组蛋白相关的文献摘要示例：
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1. **文献名称**: *"Periphilin 1 interacts with the mammary differentiation program through critical regulatory elements"*
**作者**: Smith A, et al.
**摘要**: 本研究利用重组PPHLN1蛋白探究其在乳腺上皮细胞分化中的作用，发现PPHLN1通过结合特定DNA调控元件影响分化相关基因(如KLF4)的表达，提示其可能作为表观遗传调控因子参与细胞分化过程。
2. **文献名称**: *"Recombinant PPHLN1 protein suppresses tumor growth by modulating EGFR signaling in lung cancer"*
**作者**: Chen L, et al.
**摘要**: 通过体外表达纯化PPHLN1重组蛋白，研究发现其能抑制肺癌细胞增殖并诱导凋亡，机制与干扰EGFR信号通路及下游AKT磷酸化相关，为靶向治疗提供了潜在策略。
3. **文献名称**: *"Structural and functional characterization of the PPHLN1-ADAM17 interaction in skin barrier formation"*
**作者**: Müller R, et al.
**摘要**: 利用重组PPHLN1蛋白进行结构分析，揭示了其与ADAM17蛋白酶的结合域，并通过功能实验证明该互作对表皮角质层形成至关重要，为皮肤屏障相关疾病的治疗提供分子基础。
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**说明**：
以上文献为示例，实际研究中建议通过PubMed或Web of Science以关键词“PPHLN1 recombinant protein”或“Periphilin 1 expression”检索最新论文。若研究领域较窄，可扩展至PPHLN1的功能研究文献，部分可能涉及重组蛋白实验。
**Background of PPHLN1 Recombinant Protein**
PPHLN1 (Periphilin 1) is a protein encoded by the *PPHLN1* gene, located on human chromosome 12. It plays a multifaceted role in cellular processes, including cell differentiation, proliferation, and epithelial barrier formation. Initially identified as a component of the epidermal differentiation complex, PPHLN1 is critical for skin barrier function by interacting with proteins like repetin and periplakin during cornified envelope assembly. Beyond its structural role, PPHLN1 participates in epigenetic regulation, associating with chromatin-modifying complexes such as the heterochromatin protein 1 (HP1) complex, influencing gene silencing and heterochromatin stability.
Recombinant PPHLN1 protein is engineered for research to study its molecular interactions and functional mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains bioactive properties for *in vitro* and *in vivo* studies. Its applications span cancer research, as dysregulated PPHLN1 expression correlates with tumor progression in squamous cell carcinomas and esophageal cancer, potentially serving as a biomarker or therapeutic target. Additionally, it aids in dissecting PPHLN1's role in stress responses, autophagy, and its interplay with viral pathogens.
Studies using recombinant PPHLN1 have clarified its dual roles: supporting structural integrity in epithelial tissues and modulating nuclear processes. Its involvement in diseases underscores its biomedical relevance, driving interest in therapeutic strategies targeting PPHLN1 pathways.
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