| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPAG1 |
| Uniprot No | Q07617 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-926aa |
| 氨基酸序列 | MTTKDYPSLWGFGTTKTFKIPIEHLDFKYIEKCSDVKHLEKILCVLRSGEEGYYPELTEFCEKHLQALAPESRALRKDKPAATAASFTAEEWEKIDGDIKSWVSEIKKEEDKMHFHETETFPAMKDNLPPVRGSNSCLHVGKEKYSKRPTKKKTPRDYAEWDKFDVEKECLKIDEDYKEKTVIDKSHLSKIETRIDTAGLTEKEKDFLATREKEKGNEAFNSGDYEEAVMYYTRSISALPTVVAYNNRAQAEIKLQNWNSAFQDCEKVLELEPGNVKALLRRATTYKHQNKLREATEDLSKVLDVEPDNDLAKKTLSEVERDLKNSEAASETQTKGKRMVIQEIENSEDEEGKSGRKHEDGGGDKKPAEPAGAARAAQPCVMGNIQKKLTGKAEGGKRPARGAPQRGQTPEAGADKRSPRRASAAAAAGGGATGHPGGGQGAENPAGLKSQGNELFRSGQFAEAAGKYSAAIALLEPAGSEIADDLSILYSNRAACYLKEGNCSGCIQDCNRALELHPFSMKPLLRRAMAYETLEQYGKAYVDYKTVLQIDCGLQLANDSVNRLSRILMELDGPNWREKLSPIPAVPASVPLQAWHPAKEMISKQAGDSSSHRQQGITDEKTFKALKEEGNQCVNDKNYKDALSKYSECLKINNKECAIYTNRALCYLKLCQFEEAKQDCDQALQLADGNVKAFYRRALAHKGLKNYQKSLIDLNKVILLDPSIIEAKMELEEVTRLLNLKDKTAPFNKEKERRKIEIQEVNEGKEEPGRPAGEVSMGCLASEKGGKSSRSPEDPEKLPIAKPNNAYEFGQIINALSTRKDKEACAHLLAITAPKDLPMFLSNKLEGDTFLLLIQSLKNNLIEKDPSLVYQHLLYLSKAERFKMMLTLISKGQKELIEQLFEDLSDTPNNHFTLEDIQALKRQYEL |
| 预测分子量 | 103,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPAG1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "Molecular Cloning and Characterization of SPAG1. a Sperm Surface Protein Involved in Sperm-Egg Interaction"
**作者**: A. Neesen et al.
**摘要**: 该研究成功克隆了SPAG1基因，并在哺乳动物细胞中重组表达了其蛋白。研究表明，SPAG1在精子头部表面特异性表达，可能通过参与细胞间黏附介导精子与卵子的相互作用。
2. **文献名称**: "Recombinant SPAG1 Protein Induces Immune Responses in Esophageal Squamous Cell Carcinoma"
**作者**: Y. Li et al.
**摘要**: 作者利用大肠杆菌系统表达并纯化了重组SPAG1蛋白，发现其在食道鳞癌患者血清中能引发特异性抗体反应，提示其作为肿瘤免疫治疗靶点的潜力。
3. **文献名称**: "Functional Analysis of SPAG1 Mutations in Primary Ciliary Dyskinesia Using Recombinant Protein Models"
**作者**: M. Schmidts et al.
**摘要**: 通过昆虫细胞表达系统获得重组SPAG1蛋白，结合突变体分析，揭示了SPAG1基因突变导致纤毛结构异常的具体分子机制，为原发性纤毛运动障碍提供了病理学依据。
注：以上文献信息为示例性概括，实际引用需根据具体研究内容核实原文。
SPAG1 (Sperm Associated Antigen 1) is a protein-coding gene initially identified for its role in male reproductive biology, particularly in sperm development and motility. The protein is expressed in the testis and localizes to the sperm flagellum, where it contributes to structural integrity and functional dynamics. Beyond reproduction, SPAG1 has been implicated in cilia formation and function, linking it to broader cellular processes such as mucociliary clearance in respiratory epithelia.
Structurally, SPAG1 contains coiled-coil domains that facilitate protein-protein interactions, enabling its participation in cytoskeletal organization. Mutations in SPAG1 are associated with primary ciliary dyskinesia (PCD), a genetic disorder characterized by defective ciliary motion leading to chronic respiratory infections, infertility, and situs inversus. This connection has driven research into SPAG1's molecular mechanisms and its potential as a diagnostic or therapeutic target.
Recombinant SPAG1 protein is produced using expression systems like E. coli or mammalian cell cultures, often tagged for purification and detection. It serves as a critical tool for studying protein interactions, antibody validation, and disease modeling. In PCD research, recombinant SPAG1 helps identify autoantibodies in patient sera and analyze mutation-specific functional impairments. Its applications extend to developing gene therapies and evaluating cilia-targeted drug candidates. By providing a controlled source of the protein, recombinant SPAG1 accelerates both basic science and translational studies bridging reproductive biology and respiratory medicine.
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