| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KL |
| Uniprot No | Q6UWM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-541aa |
| 氨基酸序列 | RKGSPEEASFYYGTFPLGFSWGVGSSAYQTEGAWDQDGKGPSIWDVFTHSGKGKVLGNETADVACDGYYKVQEDIILLRELHVNHYRFSLSWPRLLPTGIRAEQVNKKGIEFYSDLIDALLSSNITPIVTLHHWDLPQLLQVKYGGWQNVSMANYFRDYANLCFEAFGDRVKHWITFSDPRAMAEKGYETGHHAPGLKLRGTGLYKAAHHIIKAHAKAWHSYNTTWRSKQQGLVGISLNCDWGEPVDISNPKDLEAAERYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSLQEKSYIKGTSDFLGLGHFTTRYITERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYSVPWGFRRLLNFAQTQYGDPPIYVMENGASQKFHCTQLCDEWRIQYLKGYINEMLKAIKDGANIKGYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKASVQYYKKIIIANGFPNPREVESWYLKALETCSINNQMLAAEPLLSHMQMVTE |
| 预测分子量 | 67.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组Klotho蛋白(KL重组蛋白)的虚构参考文献示例，供参考：
1. **《Recombinant Klotho Protein Ameliorates Renal Fibrosis in Experimental Models》**
- 作者：Yamazaki, T. et al.
- 摘要：研究证明重组Klotho蛋白通过抑制TGF-β/Smad信号通路，显著减轻小鼠模型中的肾纤维化，改善肾功能，提示其治疗慢性肾脏病的潜力。
2. **《Neuroprotective Effects of Recombinant Klotho in Alzheimer’s Disease Models》**
- 作者：Kuro-o, M. et al.
- 摘要：实验显示，KL重组蛋白能减少β-淀粉样蛋白沉积，增强神经元抗氧化能力，改善阿尔茨海默病模型小鼠的认知功能，为神经退行性疾病提供新疗法。
3. **《Recombinant Klotho Attenuates Cardiovascular Remodeling in Hypertensive Rats》**
- 作者：Hu, M.C. et al.
- 摘要：通过抑制血管平滑肌细胞增殖和氧化应激，KL重组蛋白有效缓解高血压大鼠的心脏肥大和血管硬化，提示其在心血管疾病中的应用价值。
4. **《Systemic Delivery of Recombinant Klotho Enhances Lifespan and Healthspan in Mice》**
- 作者：Abraham, C.R. et al.
- 摘要：长期系统性给予KL重组蛋白延长了小鼠寿命，并改善代谢功能与骨骼健康，支持Klotho作为抗衰老靶点的潜力。
注：以上文献为示例性质，实际研究需查阅真实数据库(如PubMed)。
**Background of Recombinant KL Protein**
The recombinant KL protein refers to a genetically engineered form of the Klotho protein, initially discovered through studies on aging-related genes. The *Klotho* gene, named after the Greek goddess of fate who spins the thread of life, was first identified in mice with premature aging phenotypes. In humans, Klotho exists as two major isoforms: membrane-bound α-Klotho and secreted β-Klotho. α-Klotho, the better-characterized isoform, acts as a co-receptor for fibroblast growth factor 23 (FGF23), playing a critical role in phosphate and vitamin D metabolism, particularly in the kidneys. Beyond its endocrine functions, Klotho exhibits anti-aging properties by modulating oxidative stress, inflammation, and cellular senescence pathways.
Recombinant KL protein is typically produced using expression systems like *E. coli*, yeast, or mammalian cell cultures, enabling large-scale purification for research and therapeutic applications. Its soluble form mimics the extracellular domain of α-Klotho, retaining bioactivity in vitro and in vivo. Studies highlight its potential in treating age-associated disorders, including chronic kidney disease, neurodegenerative conditions (e.g., Alzheimer’s), and cardiovascular diseases. Preclinical models demonstrate that recombinant KL protein enhances FGF23 signaling, improves renal function, and protects against oxidative damage.
Despite promising results, challenges remain in optimizing delivery methods, stability, and tissue-specific targeting. Current research focuses on understanding its pleiotropic mechanisms and translating findings into clinical therapies, positioning recombinant KL protein as a key candidate in longevity and metabolic disease research.
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