| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSN1 |
| Uniprot No | P47710 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-185aa |
| 氨基酸序列 | RPKLP LRYPERLQNP SESSEPIPLE SREEYMNGMN RQRNILREKQ TDEIKDTRNE STQNCVVAEP EKMESSISSS SEEMSLSKCA EQFCRLNEYN QLQLQAAHAQ EQIRRMNENS HVQVPFQQLN QLAAYPYAVW YYPQIMQYVP FPPFSDISNP TAHENYEKNN VMLQW |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSN1(αs1-酪蛋白)重组蛋白的参考文献示例(文献为虚构示例，仅供格式参考，实际文献需通过学术数据库验证)：
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1. **标题**: Recombinant expression and functional analysis of CSN1 in bovine mammary epithelial cells
**作者**: Li Y, et al.
**摘要**: 研究利用哺乳动物细胞表达系统成功表达重组牛CSN1蛋白，并证明其促进乳腺细胞乳脂合成和分泌的功能，揭示了CSN1在乳蛋白合成中的调控机制。
2. **标题**: Purification and characterization of CSN1 from Escherichia coli for allergenicity studies
**作者**: Gupta R, et al.
**摘要**: 通过大肠杆菌表达体系重组生产牛源CSN1蛋白，优化纯化工艺，并验证其在乳制品致敏性检测中的应用潜力。
3. **标题**: CRISPR/Cas9-mediated CSN1 knockout in mice reveals its role in calcium homeostasis
**作者**: Wang X, et al.
**摘要**: 利用重组CSN1蛋白回补实验，在小鼠模型中证实CSN1缺失导致钙代谢异常，表明该蛋白对骨骼发育的关键作用。
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**注意**：以上文献信息为模拟生成，实际研究中请通过PubMed、Web of Science等平台检索关键词(如 "recombinant CSN1"、"alpha-S1-casein expression")。
**Background of CSN1 Recombinant Protein**
Casein alpha-s1 (CSN1) is a major component of mammalian milk caseins, constituting approximately 40% of total casein in bovine milk. As a phosphoprotein, it plays a critical role in nutrient delivery, calcium and phosphate homeostasis, and the formation of casein micelles—a unique colloidal structure essential for milk’s nutritional and physicochemical properties. CSN1 is encoded by the *CSN1S1* gene and undergoes post-translational modifications, including phosphorylation and glycosylation, which influence its functional interactions.
The recombinant production of CSN1 emerged to address challenges in studying native caseins, such as heterogeneity due to genetic polymorphisms and purification difficulties. Recombinant CSN1 is typically expressed in heterologous systems like *Escherichia coli*, yeast, or mammalian cells, enabling precise control over protein sequence and modifications. This approach facilitates research on CSN1’s structural domains, its role in micelle assembly, and interactions with other caseins or minerals.
Applications of recombinant CSN1 span food science, biotechnology, and medicine. In the dairy industry, it aids in engineering novel milk-based products with improved texture or nutritional profiles. It also serves as a model protein for studying allergenicity, lactose-free formulations, and bioactive peptide release. Beyond food, recombinant CSN1 is explored as a biomaterial for drug delivery due to its self-assembling properties and biocompatibility.
Current research focuses on optimizing expression systems to mimic native post-translational modifications and enhance yield. Challenges include maintaining solubility during bacterial expression and achieving proper folding in eukaryotic systems. Advances in synthetic biology and protein engineering are expected to further expand its utility in functional foods and therapeutic applications.
Overall, recombinant CSN1 provides a versatile tool for both fundamental research and industrial innovation, bridging gaps in understanding milk biochemistry and enabling tailored protein design.
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