| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBN3 |
| Uniprot No | Q75N90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FBN3重组蛋白的3篇参考文献及其摘要概括(注：文献为虚拟示例，实际研究可能有限)：
1. **文献名称**：*Recombinant expression and functional analysis of FBN3 in extracellular matrix remodeling*
**作者**：Chen L, et al. (2021)
**摘要**：本研究成功在HEK293细胞中表达了重组人FBN3蛋白，并发现其通过调控TGF-β信号通路参与细胞外基质的动态平衡，为FBN3相关发育异常的机制提供了新见解。
2. **文献名称**：*Structural characterization of the FBN3 N-terminal domain using recombinant protein technology*
**作者**：Wang Y, et al. (2019)
**摘要**：利用昆虫杆状病毒系统表达了FBN3的N端结构域重组蛋白，通过X射线晶体学解析其三维结构，揭示了该区域在钙离子结合和蛋白多聚化中的关键作用。
3. **文献名称**：*FBN3 knockdown and recombinant rescue in zebrafish model reveals its role in embryonic development*
**作者**：Garcia-Ruiz S, et al. (2020)
**摘要**：通过斑马鱼模型，结合重组FBN3蛋白回补实验，证明FBN3对早期胚胎神经嵴细胞迁移至关重要，其功能缺失导致心血管发育缺陷。
**备注**：FBN3研究相对较少，上述内容基于类似蛋白研究模式虚拟生成。实际文献建议通过PubMed或Web of Science以“FBN3 recombinant”等关键词检索。若需真实文献，可进一步限定时间范围或扩展至FBN1/FBN2对比研究。
Fibrillin-3 (FBN3) is a member of the fibrillin family of extracellular matrix glycoproteins, which includes FBN1 and FBN2. These proteins are essential for forming microfibrils that provide structural support to tissues and regulate growth factor signaling, particularly TGF-β. While FBN1 mutations are linked to Marfan syndrome and FBN2 to congenital contractural arachnodactyly, FBN3 remains less characterized. It is primarily expressed during embryonic development, suggesting a role in early tissue formation, though its postnatal functions are poorly understood.
Recombinant FBN3 protein refers to the engineered form produced in vitro using expression systems like mammalian cells or bacteria. This allows researchers to study its structure, interactions, and mechanisms without relying on native tissue sources. The recombinant protein retains key domains, including calcium-binding epidermal growth factor (cbEGF)-like motifs and TGF-β-binding regions, enabling investigations into its potential roles in cell adhesion, matrix assembly, or developmental pathways.
Interest in FBN3 has grown due to its speculated involvement in diseases. Some studies associate altered FBN3 expression with cancers or metabolic disorders, though evidence remains preliminary. Its restricted developmental expression also raises questions about reactivation in pathological conditions. Recombinant FBN3 tools aid in exploring these hypotheses, including antibody development, binding assays, or disease modeling. However, challenges persist in clarifying its distinct functions compared to other fibrillins and translating findings into clinical applications. Current research focuses on mapping its interactome, epigenetic regulation, and potential as a biomarker or therapeutic target.
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