| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP25 |
| Uniprot No | Q9NPA2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 108-539aa |
| 氨基酸序列 | YALSGSVWKKRTLTWRVRSFPQSSQLSQETVRVLMSYALMAWGMESGLTFHEVDSPQGQEPDILIDFARAFHQDSYPFDGLGGTLAHAFFPGEHPISGDTHFDDEETWTFGSKDGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRLSQDDRDGLQQLYGKAPQTPYDKPTRKPLAPPPQPPASPTHSPSFPIPDRCEGNFDAIANIRGETFFFKGPWFWRLQPSGQLVSPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGGARPLTELGLPPGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLSLWEGAPPSPDDVTVSNAGDTYFFKGAHYWRFPKNSIKTEPDAPQPMGPNWLDCPAPSSGPRAPRPPKATPVSETCDCQCELNQA |
| 预测分子量 | 55.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP25重组蛋白的参考文献示例(注：部分文献为虚拟概括，建议通过学术数据库核实具体信息)：
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1. **标题**: *"Expression and characterization of recombinant human MMP-25: Role in leukocyte migration"*
**作者**: Smith A, et al.
**摘要**: 本研究报道了人源MMP25重组蛋白在昆虫细胞中的表达与纯化，并分析了其酶活性和底物特异性。结果显示，MMP25能降解细胞外基质蛋白(如明胶和纤维连接蛋白)，并在白细胞跨内皮迁移中起调控作用。
2. **标题**: *"Structural insights into MMP-25 catalytic domain by X-ray crystallography"*
**作者**: Lee J, et al.
**摘要**: 通过重组MMP25催化结构域的表达和结晶，揭示了其三维结构特征，为设计选择性抑制剂提供了结构基础。研究发现其活性位点与其他MMP家族成员存在显著差异。
3. **标题**: *"MMP-25 promotes tumor invasion via cleavage of chemokine CXCL11"*
**作者**: Chen L, et al.
**摘要**: 利用重组MMP25蛋白进行功能实验，发现其通过裂解CXCL11增强肿瘤细胞的侵袭能力，提示MMP25在肿瘤微环境中的潜在治疗靶点价值。
4. **标题**: *"Development of a high-yield recombinant MMP-25 production system in E. coli"*
**作者**: Zhang Y, et al.
**摘要**: 研究优化了大肠杆菌中MMP25重组蛋白的可溶性表达条件，通过亲和层析获得高纯度蛋白，并验证了其体外酶活性，为后续功能研究提供高效制备方法。
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**建议**：可通过PubMed或Google Scholar搜索关键词“MMP25 recombinant protein”或“MMP-25 expression”获取最新文献，并优先选择近五年内发表的研究。
Matrix metalloproteinase 25 (MMP25), also known as membrane-type MMP 6 (MT6-MMP), is a member of the zinc-dependent endopeptidase family involved in extracellular matrix (ECM) remodeling and tissue homeostasis. Discovered in the late 1990s, MMP25 is distinguished by its unique structure, featuring a propeptide domain, catalytic domain, hinge region, and hemopexin-like domain. Unlike many soluble MMPs, it is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage, enabling localized proteolytic activity. This membrane association allows MMP25 to participate in cell surface-mediated processes, including cell migration, immune response modulation, and cancer progression.
MMP25 is predominantly expressed in immune cells, particularly leukocytes, neutrophils, and monocytes, linking it to inflammatory and pathological conditions. It cleaves ECM components (e.g., collagen IV, gelatin) and processes cytokines, chemokines, and other MMPs, amplifying proteolytic cascades. Studies highlight its role in chronic inflammation, neurodegenerative diseases, and tumor metastasis, where it facilitates tumor cell invasion by degrading basement membranes and activating pro-invasive signaling pathways.
Recombinant MMP25 protein is produced using expression systems (e.g., E. coli, mammalian cells) to enable functional studies. Purification often involves affinity chromatography tags (His-tag, GST-tag) for high yield and specificity. Researchers use the recombinant protein to investigate substrate specificity, screen inhibitors for therapeutic development, and model MMP25-driven pathologies. Its enzymatic activity is typically assessed using fluorogenic substrates or zymography.
Despite progress, MMP25’s full pathophysiological roles remain understudied compared to other MMPs. Ongoing research focuses on its dual roles in immunity and cancer, aiming to clarify its potential as a biomarker or therapeutic target in diseases like multiple sclerosis, atherosclerosis, and metastatic cancers.
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