| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EML2 |
| Uniprot No | O95834 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MSSFGAGKTKEVIFSVEDGSVKMFLRGRPVPMMIPDELAPTYSLDTRSELPSCRLKLEWVYGYRGRDCRANLYLLPTGEIVYFVASVAVLYSVEEQRQRHYLGHNDDIKCLAIHPDMVTIATGQVAGTTKEGKPLPPHVRIWDSVSLSTLHVLGLGVFDRAVCCVGFSKSNGGNLLCAVDESNDHMLSVWDWAKETKVVDVKCSNEAVLVATFHPTDPTVLITCGKSHIYFWTLEGGSLSKRQGLFEKHEKPKYVLCVTFLEGGDVVTGDSGGNLYVWGKGGNRITQAVLGAHDGGVFGLCALRDGTLVSGGGRDRRVVLWGSDYSKLQEVEVPEDFGPVRTVAEGHGDTLYVGTTRNSILQGSVHTGFSLLVQGHVEELWGLATHPSRAQFVTCGQDKLVHLWSSDSHQPLWSRIIE |
| 预测分子量 | 61.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EML2重组蛋白的示例参考文献(请注意，这些为模拟示例，非真实文献，仅供格式参考)：
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1. **文献名称**：*Structural characterization of the EML2 protein and its role in microtubule dynamics*
**作者**：Smith J, et al.
**摘要**：解析了EML2重组蛋白的晶体结构，发现其N端WD40结构域对微管结合至关重要，并揭示了其在细胞有丝分裂中调控微管稳定的机制。
2. **文献名称**：*EML2 overexpression promotes cancer cell invasion via MAPK signaling pathway*
**作者**：Chen L, et al.
**摘要**：通过重组EML2蛋白体外实验，证明其过表达激活MAPK通路，增强肺癌细胞迁移能力，提示其作为潜在肿瘤治疗靶点。
3. **文献名称**：*High-yield expression and purification of recombinant EML2 in E. coli for drug screening*
**作者**：Garcia R, et al.
**摘要**：开发了一种大肠杆菌表达系统，高效纯化功能性EML2重组蛋白，并应用于小分子抑制剂筛选，发现两种候选化合物可抑制EML2-微管相互作用。
4. **文献名称**：*EML2 gene cloning and functional analysis in Drosophila model*
**作者**：Kim S, et al.
**摘要**：克隆了果蝇EML2同源基因，利用重组蛋白证实其参与神经元轴突导向，突变体表现出显著的发育缺陷。
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**建议**：实际研究中请通过PubMed、Web of Science等平台，以“EML2 recombinant protein”“EML2 function”等关键词检索最新文献，或查阅EML家族蛋白的综述(如《Current Opinion in Structural Biology》相关文章)获取权威信息。
EML2 (Echinoderm Microtubule-Associated Protein-Like 2) is a member of the conserved EML family of microtubule-binding proteins implicated in regulating mitotic spindle dynamics, cell cycle progression, and ciliary assembly. First identified through homology with the sea urchin EMAP protein, human EML2 contains characteristic N-terminal tandem WD40 repeat domains and a C-terminal EML homology (EH) domain, also called the EMAP-like domain. These structural features enable interactions with microtubules and other signaling proteins, such as Aurora kinases, suggesting roles in mitotic checkpoint control and chromosome segregation.
Recombinant EML2 proteins are engineered for functional studies and therapeutic exploration. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), recombinant EML2 retains microtubule-stabilizing properties and serves as a tool to investigate its molecular mechanisms. Studies reveal that EML2 regulates microtubule dynamics by suppressing catastrophe events and promoting polymerization, which is critical for maintaining mitotic spindle integrity. Dysregulation of EML2 has been linked to cancer progression, ciliopathies, and developmental disorders, emphasizing its biomedical relevance.
Recent research highlights EML2's involvement in primary cilia formation, where it facilitates trafficking of ciliary components along microtubules. Its recombinant forms are also used to study interactions with cancer-associated kinases or ciliary proteins, aiding drug discovery. Despite progress, questions remain about tissue-specific isoforms, post-translational modifications, and precise roles in disease pathways. Recombinant EML2 thus represents a key reagent for dissecting microtubule-related cellular processes and developing targeted therapies.
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