| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYH14 |
| Uniprot No | Q7Z406 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYH14重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Expression and functional characterization of MYH14 in neuronal cells"*
**作者**：Li, X., et al.
**摘要**：本研究成功在大肠杆菌中重组表达了人源MYH14蛋白，并验证其在神经元细胞中的定位与功能，发现其通过调控细胞骨架动态影响轴突生长。
2. **文献名称**：*"Structural insights into MYH14 mutations associated with hearing loss"*
**作者**：Zhang, Y., et al.
**摘要**：通过体外重组表达MYH14突变体蛋白，结合冷冻电镜分析，揭示了特定突变导致蛋白构象异常，破坏内耳毛细胞肌动蛋白网络，从而引发遗传性耳聋的分子机制。
3. **文献名称**：*"Development of a MYH14-based in vitro motility assay system"*
**作者**：Tanaka, H., et al.
**摘要**：报道了一种利用重组MYH14蛋白构建的体外运动功能检测平台，证实其与F-actin结合后产生的机械力特性，为研究肌球蛋白家族功能提供了新工具。
备注：上述文献为模拟示例，实际研究中请通过PubMed或Web of Science检索真实发表的论文。若需具体文献，建议结合关键词"MYH14 recombinant protein"或"MYH14 expression"进行精确查询。
The MYH14 recombinant protein is derived from the MYH14 gene, which encodes the heavy chain of non-muscle myosin II (NMMHC-II), a member of the myosin superfamily involved in cellular motility, cytokinesis, and structural maintenance. MYH14. also known as myosin heavy chain 14. is one of three isoforms (IIA, IIB, and IIC) in the non-muscle myosin II group. It plays a critical role in regulating actin cytoskeleton dynamics, essential for processes like cell migration, adhesion, and intracellular transport. Unlike muscle-specific myosins, MYH14 is ubiquitously expressed, with notable roles in neural, smooth muscle, and auditory systems. Mutations in MYH14 have been linked to autosomal dominant hearing loss (DFNA4) and peripheral neuropathy, highlighting its importance in cellular and tissue homeostasis.
Recombinant MYH14 protein is typically produced using expression systems (e.g., bacterial, insect, or mammalian cells) to enable functional studies. Its structure includes an N-terminal motor domain for actin binding and ATP hydrolysis, a regulatory light chain-binding neck region, and a C-terminal coiled-coil tail mediating filament assembly. Researchers use recombinant MYH14 to investigate its enzymatic activity, interactions with cytoskeletal components, and pathological mechanisms caused by mutations. Studies focus on how MYH14 dysfunction disrupts cellular mechanics, leading to defects in neural development, muscle contraction, or auditory function. Its recombinant form also aids in drug screening for disorders associated with myosin dysregulation. Despite progress, MYH14's isoform-specific roles remain less characterized compared to MYH9 or MYH10. necessitating further exploration of its tissue-specific functions and therapeutic potential.
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