| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYH7B |
| Uniprot No | A7E2Y1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYH7B重组蛋白的3篇代表性文献的简要概述(注：文献为模拟示例，实际引用需核实)：
1. **文献名称**：*Recombinant MYH7B Protein Expression and Its Role in Muscle Function*
**作者**：Smith J, et al.
**摘要**：本研究成功构建了MYH7B基因的重组表达载体，并在HEK293细胞中实现了可溶性表达。纯化后的重组蛋白通过体外实验证实了其在调节骨骼肌收缩中的潜在作用，为研究肌纤维疾病提供了新工具。
2. **文献名称**：*Structural Characterization of MYH7B Recombinant Protein Using Cryo-EM*
**作者**：Li X, et al.
**摘要**：通过冷冻电镜技术解析了重组MYH7B蛋白的三维结构，揭示了其ATP酶活性区域的关键构象变化，为理解该蛋白在心肌病相关突变中的功能异常机制提供了结构基础。
3. **文献名称**：*Functional Analysis of MYH7B Mutants via Recombinant Protein Assays*
**作者**：Garcia R, et al.
**摘要**：利用重组MYH7B蛋白系统分析了三种临床突变体(如R723C)的ATP水解活性变化，发现突变导致肌球蛋白动力受损，解释了其与遗传性肥厚型心肌病的关联性。
(提示：实际文献检索建议使用PubMed/Google Scholar，以“MYH7B recombinant”“MYH7B expression”等关键词筛选近年研究。)
The MYH7B recombinant protein is derived from the MYH7B gene, which encodes a member of the myosin heavy chain (MyHC) family, specifically classified under class II myosins. Myosins are motor proteins critical for muscle contraction, intracellular transport, and cellular motility. MYH7B is structurally similar to MYH7 (β-myosin heavy chain), a well-characterized cardiac/slow-twitch skeletal muscle protein, but it exhibits distinct expression patterns and regulatory mechanisms. While MYH7 is predominantly expressed in adult heart and slow skeletal muscle, MYH7B is found in cardiac tissue, skeletal muscle, and certain non-muscle cells, suggesting broader functional roles.
The MYH7B protein consists of a heavy chain with conserved domains: an N-terminal motor domain (ATPase activity), a neck region bound to light chains, and a C-terminal tail involved in filament assembly. Recombinant MYH7B is typically produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells) for biochemical and functional studies. Its production enables researchers to investigate its role in sarcomere organization, contractile dynamics, and interactions with other muscle-related proteins like actin and titin.
Emerging studies suggest MYH7B may regulate cardiac hypertrophy and muscle adaptation under stress. Notably, alternative splicing generates isoforms with divergent C-terminal tails, potentially influencing cellular localization and function. Dysregulation of MYH7B has been tentatively linked to cardiomyopathies and muscular disorders, though its exact pathological mechanisms remain unclear. Recombinant MYH7B serves as a vital tool for dissecting these mechanisms, screening therapeutic compounds, and modeling disease-associated mutations. Current research focuses on clarifying its physiological significance compared to MYH7 and exploring its therapeutic potential in muscle-related diseases.
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